Expression of SNRPA and related molecular mechanisms in gastric cancer
10.13315/j.cnki.cjcep.2025.07.003
- VernacularTitle:胃癌中SNRPA的表达及相关分子机制
- Author:
Sijia WANG
1
;
Min CUI
;
Bingmei LI
;
Meichen TONG
;
Na ZHOU
;
Qian ZHANG
;
Zhang CAO
Author Information
1. 滨州医学院病理学教研室,烟台 264003
- Publication Type:Journal Article
- Keywords:
gastric neoplasms;
SNRPA;
prognosis;
proliferation;
invasion
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(7):853-861,867
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of SNRPA in gastric cancer and its effect on the proliferation,migration and invasion of gastric cancer cells.Methods The expression of SNRPA in gastric cancer tissues was ana-lyzed using The Cancer Genome Atlas(TCGA)database.The level of SNRPA was detected by immunohistochemistry(EnVision method),and its relationship with clinicopathological parameters was analyzed.Western blot was used to detect the expression of SNRPA in 15 cases of fresh gastric cancer and paracancerous tissues.The expression of SNRPA in MGC-803 and GES-1 cells was detected by immunofluorescence staining.The effects of SNRPA expression on the proliferation,migration and invasion of gastric cancer cell lines were determined by RNA interference technology and cell function assays,and the expression levels of Cyclin D1 protein and EMT-related proteins(E-cadherin,N-cadher-in)were detected by Western blot.Results The expression level of SNRPA in gastric cancer tissues was significantly higher than that in normal gastric mucosal tissues(P＜0.05),with an area under the curve(AUC)of 0.902 for diag-nostic accuracy.The Kaplan-Meier survival curves showed that the survival time of the group with high expression of SNRPA was shorter.SNRPA expression correlated with tumor size,Ki67,infiltration depth,and p53 status(P＜0.05).Compared with the corresponding control group,SNRPA silencing inhibited the proliferation,migration and in-vasion ability of gastric cancer cells,accompanied by decreased Cyclin D1 and N-cadherin expression and increased E-cadherin expression(P＜0.05).Conclusion SNRPA expression is upregulated in gastric cancer tissues and cell lines,promoting the proliferation,migration,and invasion of gastric cancer cells,and may be a potential molecular marker for gastric cancer.
