Isolation,identification and characterization of a phage of Clostridium perfrin-gens
10.16303/j.cnki.1005-4545.2025.09.11
- VernacularTitle:1株产气荚膜梭菌噬菌体的分离鉴定及特性分析
- Author:
Guang ZHU
1
;
Yunzhou WANG
;
Maishou ZHANG
;
Xueting WANG
;
Jing SUN
;
Jiacai WANG
Author Information
1. 山东畜牧兽医职业学院,山东潍坊 261000
- Publication Type:Journal Article
- Keywords:
bovine necrotic enteritis;
Clostridium perfringens;
phage
- From:
Chinese Journal of Veterinary Science
2025;45(9):1902-1908
- CountryChina
- Language:Chinese
-
Abstract:
In order to the phage therapy for Clostridium perfringens(Cp),Cp was used as the host bacterium to screen specific phages from sewage collected from a large-scale beef cattle farm in a region of Shandong Province.Transmission electron microscopy was utilized to observe the mor-phological structure of the phages.Their characteristics were analyzed through the optimal multi-plicity of infection(MOI),host range,one-step growth curve,and antibacterial experiments.The re-sults showed that one phage was successfully isolated from 18 sewage samples.The plaque was transparent without a halo,exhibiting a broad host range and no lytic reaction with other tested bacteria.The optimal MOI was 0.1,and the phage entered the burst phase in 30 min.The phage maintained high titers at pH3-13 and remained active after treatment at 60℃ for 60 min,demon-strating good stability.Mice were infected with CP and treated with bacteriophage.Animal experi-ments showed that the isolated bacteriophage had a significant inhibitory effect on CP.The results indicated that the isolated phage,designated vB-CpP-Bp7,was a virulent phage with excellent lytic capability,acid-base stability,and thermal stability,laying a foundation for further application of this phage in phage therapy.
