Effcts of HBO on the expression of AQP-4 and perfusion following cerebral blast injury in rabbits
10.3760/cma.j.issn.1009-6906.2012.03.010
- VernacularTitle:高压氧暴露对兔脑爆炸伤后脑组织水通道蛋白4的表达及血液灌注的影响
- Author:
Jia-chuan LIU
1
;
Wen-jiang SUN
;
Yong-ming ZHANG
;
Yan-yan YANG
;
Hong TANG
;
Bing-cang LI
;
Liang-chao ZHANG
Author Information
1. 解放军第一○五医院神经外科, 合肥,230031
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Blast injury;
Aquaporins-4;
Perfusion weighted imaging
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2012;19(3):163-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective Mechanism of cerebral edema following blast injury in rabbits through the study of the expression of aquapodins-4 ( AQP-4 ) introduced by HBO,and perfusion as well,after cerebral blast injury.Methods One hundred and fifty New Zealand rabbits were randomly divided into the treatment group,the non-treatment group and the control group ( 10 animals).The animal model of cerebral blast injury was developed by using paper detonator.The treatment and the non-treatment groups were randomly divided into the 1 h,6 h,12 h,24 h,72 h,7 d and 14 d sub-groups,each consisting of 10 animals.The expression of AQP-4 mRNA was measured with RT-PCR,and the perfusion of the brain tissue was detected with perfusion weighted imaging (PWI) at each time points,following cerebral injury.Results The expression of AQP-4 mRNA began to increase 1 h after injury,reached peak in 72 h,and then decreased at day 7.Following initial HBO treatment,the expression of AQP-4 mRNA significantly decreased at various time points after 6 hours (P ＜0.05).PWI test indicated that blood perfusion for the non-treatment group significantly decreased in the initial stage of injury,however,12 h after injury perfusion gradually increased,and the amount of perfusion (close to the control group) was maintained for 2 weeks.For the treatment group,perfusion decreased more significantly in the early stages of injury (1-6 h).Conclusions Early HBO therapy could enhance the partial pressure of oxygen in the affected brain tissue,increase blood oxygen content and alleviate secondary cerebral edema induced by cerebral ischemia.
