LAMB1 regulates the expression of glutamate receptors in mouse cerebral cortical neurons through the ERK/F-actin pathway
10.16557/j.cnki.1000-7547.2025.05.001
- VernacularTitle:LAMB1通过ERK/F-actin通路调控小鼠大脑皮质神经元中谷氨酸受体的表达
- Author:
Zhenzhen LI
1
;
Kexin LIU
;
Wanneng LIU
;
Zhiwei DOU
;
Shuai WANG
;
Yang TANG
;
Ceng LUO
;
Shengxi WU
Author Information
1. 空军军医大学基础医学院神经生物学教研室,西安 710032
- Publication Type:Journal Article
- Keywords:
cortical neurons;
laminin β1(LAMB1);
fibrous actin(F-actin);
ERK pathway;
glutamate receptors;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(5):549-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role and molecular mechanism of laminin β1(LAMB1)in cortical neurons in regulation of glutamate receptors.Methods:Recombinant lentivirus(LV-shLamb1)-mediated knockdown of LAMB1 expression in mouse primary cortical neurons was performed,followed by immunofluorescence staining and Western blot to detect changes in F-actin,glutamate receptor subtypes(AMPA receptors GluR1/GluR2,NMDA receptors NR1/NR2A),and ERK-related protein expression in cortical neurons.Results:LV-shLamb1 significantly inhibited LAMB1 expression in mouse cortical neurons.Concurrently,LV-shLamb1 markedly increased F-actin polymerization,as well as the expression of AMPA receptor subunits GluR1 and GluR2,and NMDA receptor subunits NR1 and NR2A.Further,Western blot detection showed that the phosphorylation level of ERK was significantly increased after LV-shLamb1 infec-tion.Conclusion:LAMB1 is expressed in cortical neurons.Suppression of LAMB1 expression in mouse cortical neu-rons activates the ERK pathway,which in turn promotes the polymerization of the cytoskeletal protein F-actin and the expression of glutamate receptors.This suggests that LAMB1 may regulate F-actin homeostasis and glutamate receptor levels through the ERK pathway,thereby playing a potentially important role in neuronal function.
