Exercise-induced chronic fatigue reduces spatial recognition and memory ability and the expression of parvalbumin in the hippocampal DG region of rats
10.16557/j.cnki.1000-7547.2025.05.003
- VernacularTitle:运动疲劳降低大鼠的空间识别记忆能力及海马DG区小清蛋白的表达
- Author:
Hongyang LI
1
;
Lequan LIN
;
Chang SU
;
Qianyun YANG
;
Shaoju ZENG
;
Chao XI
;
Lina SUN
Author Information
1. 北京师范大学体育与运动学院,北京 100875
- Publication Type:Journal Article
- Keywords:
exercise-induced chronic fatigue;
dentate gyrus(DG);
spatial recognition and memory;
parvalbumin-positive interneurons(PV-INs);
astrocytes(AS);
calcium/calmodulin-dependent protein kinase Ⅱ(CaMK Ⅱ);
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(5):565-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the neural regulatory mechanism of parvalbumin-positive interneurons(PV-INs)in the dentate gyrus(DG)involved in the impairment of spatial recognition memory by exercise-induced chronic fatigue.Methods:Male Sprague-Dawley(SD)rats were divided into control group and fatigue group by random num-ber method.A three-level incremental load treadmill training program was selected to establish a chronic exhaustion exercise fatigue model.The spatial recognition memory ability of rats was tested by novel object recognition test.The ac-tivation levels and quantitative changes of astrocytes(AS)and PV-INs in the DG region was observed and quantified through immunofluorescence staining and immunohistochemical staining.The phosphorylation level of calcium/calmodu-lin-dependent protein kinase Ⅱ(CaMK Ⅱ)in the hippocampus was detected by Western blot.Results:In the test of novel object recognition,the exploration time of novel object was reduced in the fatigue group,and the discrimination index was significantly lower than that in the control group(P＜0.01).Immunohistochemical staining showed that PV-INs in the DG region of fatigue rats were lighter and fewer than those in the control group,the fibers were short and sparse,and the positive cell density and average optical density of cells were significantly lower than those in the control group(P＜0.01).Immunofluorescence staining showed that AS was significantly activated,glial fibrillary acidic pro-tein(GFAP)was stained deeply,and the cell processes were dense and elongated in the DG region of fatigue rats.The positive cell density and mean fluorescence intensity were significantly higher than those of the control group(P＜0.01).The results of Western blot showed that the phosphorylation level of CaMK Ⅱ protein in the hippocampus of the fatigue group was significantly reduced than that of the control group(P＜0.01).Conclusion:Exercise-induced chro-nic fatigue inhibited PV-INs in the rat hippocampal DG region.The excessive activation of AS following exercise fatigue may be a major contributor to this PV-INs suppression.Concurrently,reduced phosphorylation levels of CaMK Ⅱ protein were observed in hippocampal tissue.These alterations ultimately impaired spatial recognition memory in the rats.
