Effect of simulated saturation diving on immune function of T lymphocyte in mice

VernacularTitle:模拟氦氧饱和潜水环境对小鼠T细胞免疫功能的影响
Author: Heng-rong YUAN ¹ ; Yi-qun FANG ; Rui-yong CHEN ; Ling-zhen ZHANG ; Zheng MA ; Hai-tao WANG ; Miao MENG
Author Information

1. 第二军医大学,上海,200433
Publication Type:Journal Article
Keywords: Saturation diving; T lymphocyte; Flow cytometry; Cell immunity
From: Chinese journal of nautical medicine and hyperbaric medicine 2008;15(2):78-80
CountryChina
Language:Chinese
Abstract: Objective To investigate effect of simulated saturation diving on immune function of T lymphocyte in mice.Methods Thirty Kunming-race mice were randomly divided into 5 groups:control group,at 100 m simulated saturation diving exposure for 24 h and 72 h groups,at 400 m simulated saturation diving exposure for 24 h and 72 h groups,each group for 6 mice.The T lymphocyte subsets of peripheral blood,collected 10 minutes after exposure,were assayed by flow cytometry.Results The level of CD4+ lymphocyte and CD4+/CD8+ ratio tended to increase after exposure at 100 m,while the levels of CD4+ lymphocyte and CD4+/CD8+ significantly decreased after exposure at 400 m.Conclusions The effect of simulated saturation diving on cellular immune function was correlated significantly with exposed pressure.