Changes in the content of monocyte chemoattractant protenin-1 in aqueous humor following penetrating corneal trauma complicated with seawater immersion in rabbits
10.3760/cma.j.issn.1009-6906.2009.06.006
- VernacularTitle:兔角膜穿通伤合并海水浸泡后房水单核细胞趋化蛋白-1含量变化的研究
- Author:
Jing ZUO
1
;
Qin-hua FAN
1
;
Jian-dong YANG
1
;
Yu LIU
1
;
Zhi-qiang ZHANG
1
;
De-kui REN
1
Author Information
1. 解放军第八一医院眼科,江苏省南京,210002
- Publication Type:Journal Article
- Keywords:
Penetrating corneal trauma;
Seawater immersion;
MCP-1;
Aqueous humor
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2009;16(6):337-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate changes in the content of monocyte chemoattractant protenin-1 (MCP-1) in aqueous humor following penetrating corneal trauma induced by explosion and complicated with seawater immersion in rabbits, and also its importance in the mechanism involved. Methods The model of penetrating corneal trauma was developed with fire crackers by using 20 rabbits as subjects, with the right eye as the experimental eye and left eye as the control. The fight eye was immersed with seawater and the left eye immersed with saline solution. Levels of MCP-1 in aqueous humor for the 2 groups were determined by enzyme -linked immunosorbent assay (ELISA) on the 1st, 2nd, 3rd, 5th and 7th days following development of the model. Results Levels of MCP-1 in aqueous humor for the experimental group were 247.018 ng/L, 143.939 ng/L, 93.637 ng/L respectively on the 1st, 2nd, 3rd days following development of the model. Comparisons showed that statistical difference could be seen between the 2 groups (P <0.01). Conclusions Levels of MCP-1 in aqueous humor following penetrating corneal trauma by explosion and complicated with seawater immersion were significantly higher than those of the control group, indicating that MCP-1 might be involved and have an important role to play in this pathological process.
