Mechanism of Banxia-Xiexin decoction activating Hippo/YAP signaling pathway to alleviate chronic atrophic gastritis
- VernacularTitle:半夏泻心汤激活Hippo/YAP信号通路减轻慢性萎缩性胃炎
- Author:
Jia-min HU
1
;
Yu-jie SUN
1
;
De-bin HUANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Banxia-xiexin decoction; chronic atrophic gastritis; Hippo signaling pathway; inflammatory fac-tors; pepsinogen; gastrin-17
- From: Chinese Pharmacological Bulletin 2025;41(8):1578-1583
- CountryChina
- Language:Chinese
- Abstract: Aim To clarify the protective effect of Banxia-Xiexin decoction(BD)on chronic atrophic gastritis(CAG).Methods After establishing a CAG model,BD was used for 4 weeks.The pathological changes in the gastric mucosa were observed through HE staining,while serum biomarkers were detected u-sing ELISA.The expression of key factors in the Hip-po/YAP signaling pathway was examined by RT-qPCR and Western blot.Finally,the expression of YAP and p-YAP proteins was detected by immunefluorescence.Results After BD intervention,the thickness of the gastric mucosa layer,intestinal metaplasia,and inflam-mation in CAG rats showed significant dose-dependent improvement.BD could reduce the expression of IL-6,IL-1β,and TNF-α in the serum of CAG rats,increase the expression of IL-10,and elevate PGR and G-17 lev-els.Following BD intervention,YAP1 was largely phosphorylated and inactivated,with significant increa-ses in p-YAP/YAP protein expression levels and fluo-rescence intensity.Additionally,the protein and mRNA expression levels of MST1 and LATS1 were elevated,indicating the activation of the Hippo/YAP signaling pathway.Conclusions BD exerts a significant phar-macological effect in the treatment of CAG,and its mechanism may be related to the upregulation of MST1-LATS1 protein expression,leading to the inacti-vation of YAP through phosphorylation.
