Disruption of mouse's microfilament cytoskeleton induced by simulated microgravity on alkaline phosphatase and osteocalcin
10.3760/cma.j.issn.1007-6239.2011.02.004
- VernacularTitle:模拟微重力诱导小鼠成骨细胞微丝变化对碱性磷酸酶及骨钙蛋白的影响
- Author:
Mai-tao WANG
1
;
Zhen HUANG
;
Li YANG
;
Hong CHEN
;
Jia-lin SU
;
Yan-xing MAI
;
Rui YANG
;
Huan-cheng ZHOU
Author Information
1. 南方医科大学附属珠江医院内分泌科,广州,510282
- Publication Type:Journal Article
- Keywords:
Weightlessness simulation;
Osteoblast;
Cytoskeleton;
Alkaline
- From:
Chinese Journal of Aerospace Medicine
2011;22(2):97-101,封2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the effects of the mouse's microfilament cytoskeleton change induced by simulated microgravity on alkaline phosphatase (ALP) and osteocalcin (OCN), and then to explore the mechanism of weightlessness leading osteoporosis. Methods Research object was the osteoblast-like cell line of mouse (MC3T3-E1). Microgravity was simulated by rotary cell culture system. Cells were divided into four groups: simulated microgravity group, 0.5 μg/ml cytochalasin B group, simulated microgravity+0.5 μg/ml cytochalasin B group and normal gravity group. Cells had been cultivated for 48 h when immunofluorescence staining was made by fluoresceine isothiocyanate phalloidin (FITC-phallacidin). Then the changes of cell microfilament were observed by laser confocal microscope. At same time, the activity of ALP in the cell culture medium was tested by ALP detecting reagent, and the expression of OCN in cell lysate was measured with ELISA method. Results The microfilament cytoskeleton depolymerization, reduction of tension fibers and disorder were observed in all but normal gravity group, especially in the simulated microgravity+0.5 μg/ml cytochalasin B group. Compared with normal gravity group, the activity of ALP and the expression of OCN in other groups were in decreased tendency (F=17.23, 121.91, P<0.01), of which significant difference was found between simulated microgravity+0.5 μg/ml cytochalasin B group and 0.5 μg/ml cytochalasin B group (P<0.05). Conclusion Microgravity affects the activity of ALP and the expression of OCN and these may be associated with the disruption of microfilament cytoskeleton that induced by simulated microgravity.
