Effect of hyperbaric oxygen on intestinal mucosa injury of severe burn rats
10.3760/cma.j.issn.1009-6906.2011.04.009
- VernacularTitle:高压氧治疗对严重烧伤大鼠肠黏膜屏障作用的影响
- Author:
Ying YAN
1
;
Guo-yu HUANG
;
Dao-feng BEN
;
Yi-tao JIA
Author Information
1. 解放军第四一三医院烧伤科,浙江省舟山,316000
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Bum injury;
Intestinal mucosa;
Immune function;
Diamine oxidase;
D-lactate
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2011;18(4):217-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of hyperbaric oxygen (HBO) on intestinal mucosa injury of severe burn rats. Methods Forty-five male healthy adult SD rats were randomly divided into 3 groups: the sham group(n = 16), the burn injury group(n = 14), and burn injury + HBO group(n = 15). The animals in burn injury groups sustained 3-degree burn injury on the back with a total body surface area (TBSA) of 30％.Twenty-four hours after bum injury, those in the HBO group were exposed to 95％ oxygen at a pressure of 0. 15MPa, 1 hour a day, for a succession of 5 days. On the 7th day after bum injury, blood samples from the intestinal mucosa and inferior vena cava were collected to detect differences in pathological changes in the intestinal mucosa of various groups, and serum levels of diamine oxidase (DAO) and D-lactate were also measured. Results When compared with the sham group, significant pathological changes in the intestinal mucosa could clearly be seen in the bum injury group. Following exposure to HBO, lesion in the intestinal mucosa improved obviously. On the 7th day after bum injury, levels of DAO and D-lactate increased significantly. However, after HBO treatment, the levels of DAO and D-lactate decreased significantly. Conclusions HBO seemed to have the effect of protecting the barrier of intestinal mucosa in severe burn rats.
