Determination of 9 synthetic cannabinoids in e-cigarette oil by liquid chromatography-high resolution mass spectrometry
10.13618/j.issn.1001-5728.2025.02.014
- VernacularTitle:液相色谱-高分辨质谱法测定电子烟油中9种合成大麻素
- Author:
Chunyan XIN
1
;
Yu FANG
;
Shaohua FANG
;
Ziyuan YANG
;
Qianqian GUO
;
Sicui JIANG
Author Information
1. 上海道格仕医疗器械有限公司,上海 201702
- Publication Type:Journal Article
- Keywords:
High resolution mass spectrometry;
E-cigarette oil;
Synthetic cannabinoids
- From:
Chinese Journal of Forensic Medicine
2025;40(2):207-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study established a qualitative and quantitative detection method for 9 new synthetic cannabinoids in e-cigarette oil using liquid chromatography-high resolution mass spectrometry.Methods E-cigarette oil was extracted with methanol,concentrated by nitrogen blowing under a water bath at 60℃,and separated using an Acquity UPLC? HSS T3(100 mm×2.1 mm,1.8 μm)column.Gradient elution was performed with ammonium acetate buffer solution(20 mmol/L)containing 0.1%formic acid and acetonitrile as the mobile phase.Detection was carried out in the positive ion mode of the HESI ion source using DDA(FullScan+dd-MS2)mode.Quantification was performed using the external standard method based on precise precursor ion peak areas,while qualitative analysis relied on retention times and characteristic fragment ions.Results The results showed that the standard curves of all 9 synthetic cannabinoids in blank e-cigarette oil matrix exhibited good linearity with R2 values>0.999.The limits of detection ranged from 0.0001 to 0.01 μg/g,and the limits of quantification ranged from 0.0003 to 0.03 μg/g.Recovery rates at low,medium,and high spiking levels in blank e-cigarette oil matrix ranged from 75.7%to 115%,with relative deviations<8.5%.Conclusion This method features a short instrument detection time of only 10 min,relatively simple operation,low detection limits,high accuracy,and good precision,making it applicable for rapid screening and quantitative analysis of synthetic cannabinoids in e-cigarette oil.
