Leptin promotes breast cancer cell MCF-7 migration and invasion through inhibiting ACSL5
- VernacularTitle:瘦素通过抑制ACSL5促进乳腺癌细胞MCF-7的迁移和侵袭
- Author:
Tao ZENG
1
;
Lan WEI
;
Yong-zhu XU
;
Shi-yu YANG
;
Hao-li SUN
;
Ting-ting DANG
;
Yi-qing YOU
;
Jia-feng TANG
;
Yan ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: breast cancer; leptin; ACSL5; migration; invasion; EMT
- From: Chinese Pharmacological Bulletin 2025;41(4):654-660
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the possible regulatory effect of leptin on acyl-CoA synthetase long chain fami-ly member ACSL5 and their effect on migration and in-vasion of breast cancer cell,and to explore the underly-ing mechanism.Methods The expression of leptin receptor was detected by immunofluorescence assay.The migration and invasion ability of MCF-7 cells were detected by wound healing assay and Transwell assay respectively.The downstream target gene of leptin was analyzed by PCR microarray data.The expression of ACSL5 in breast cancer and its correlation with the staging and prognosis of breast cancer patients were as-sessed uing bioinformatics methods.The expression of ACSL5 in MCF-7 cells treated with different concentra-tions of leptin was detected using real time fluorescence quantitative polymerase chain reaction(RT-qPCR).Overexpressing ACSL5 was constructed by lentiviral transfection;the expressions of EMT related proteins,AMPK-α and p-AMPK-α were detected by Western blot.Results Leptin promoted breast cancer cell mi-gration and invasion and EMT.ACSL5 was significant-ly low expressed in breast cancer and related to progno-sis.Leptin downregulated the expression of ACSL5 through OBR.Leptin activated AMPK pathway to downregulate ACSL5 and promote migration,invasion and EMT of breast cancer cells.Conclusions Leptin may promote the migration,invasion and EMT of breast cancer by downregulating ACSL5 through activating AMPK pathway.
