Construction and evaluation of hepatocellular carcinoma models in mice with different immune microenvironments
10.13929/j.issn.1672-8475.2025.04.007
- VernacularTitle:构建与评价不同免疫微环境小鼠肝癌模型
- Author:
Yujie ZHONG
1
;
Yuyang DAI
1
;
Shijie FU
1
;
Kanglian ZHENG
1
;
Chaofan ZHU
1
;
Guang CAO
1
;
Liang XU
1
;
Chuanxin NIU
1
;
Xiaoyu FAN
1
;
Xiaodong WANG
1
Author Information
1. 北京大学肿瘤医院暨北京市肿瘤防治研究所介入治疗科 恶性肿瘤发病机制及转化研究教育部重点实验室,北京 100142
- Publication Type:Journal Article
- Keywords:
liver neoplasms,experimental;
mice;
tumor microenvironment;
immunotherapy;
animal experimentation
- From:
Chinese Journal of Interventional Imaging and Therapy
2025;22(4):260-266
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct mice hepatocellular carcinoma models with different tumor immune microenvironments(TIME)and explore the differences.Methods H22 and hepa1-6 were used to construct subcutaneous transplantation tumor model of C57 mice as homologous hepatocellular carcinoma cell lines(denoted as H22 group and hepal-6 group,each n=8),and the differences of TIME were evaluated.Immunohistochemistry was used to detect and quantify the infiltration of T cells,CD4+T cells,CD8+T cells,regulatory T cells and B cells in TIME.Flow cytometry was performed to detect the differences of composition of immune cell subpopulations in peripheral blood and tumor parenchyma.Gene expression profile characteristics of tumor tissue were analyzed based on high-throughput transcriptome sequencing technology,and enrichment analyses of immune-related signaling pathways were evaluated combined with gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG).Results H22 group showed cold and hepa1-6 group showed hot TIME characteristics.The number of T cells,CD4+T cells and CD8+T cells in tumor tissue of H22 group were all lower,while the proportion of T cells,CD4+T cells and CD8+T cells in peripheral blood were all higher than those of hepa1-6 group(all P＜0.05).Compared with H22 group,up-regulated genes of tumor tissue in hepa1-6 group expressed significantly enriched in tumor immune activation-related signaling pathways.Conclusion H22 and hepa1-6 hepatocellular carcinoma models showed distinct TIME characteristics of cold and hot tumors,respectively,and the amount of immune cells in tumor tissue of the former were significantly lower than those in the latter.
