The protective effect of Gualou Guizhi granules on neuronal injury induced by LPS-activated microglia based on Notch signaling pathway
- VernacularTitle:基于Notch信号通路探讨栝楼桂枝颗粒对脂多糖活化小胶质细胞诱导神经元损伤的保护作用
- Author:
Xue-zhen LI
1
;
Xiao-xue ZOU
1
;
Wen-ting CHEN
1
;
Yi FENG
1
;
Ya-nan LI
1
;
Yu-qin ZHANG
1
;
Li-hong NAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Gualou Guizhi granules; lipopolysaccha-ride; microglia; Notch signaling pathway; neuroin-flammation; HT22 cells
- From: Chinese Pharmacological Bulletin 2025;41(4):781-786
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effect of Gualou Guizhi granules(GLGZG)on neuronal injury induced by LPS-activated microglia based on Notch signaling pathway.Methods LPS-activated microglia were co-cultured with neurons to construct neuron inju-ry models,and the cells were divided into the control group,model group,Notch inhibitor(DAPT)group,GLGZG(50,100,200 mg·L-1)group,DAPT+100 mg·L-1GLGZG group.After intervention,the activity of HT22 cells was detected by CCK-8 method,and rel-ative mRNA expression was detected by real-time PCR.The relative protein expression was detected by Western blot.Results Compared with the model group,after GLGZG intervention,the cell activity was significantly improved,GLGZG decreased IL-6,IL-12,Bax,Notch 1,caspase-3,Delta-1,NICD,RBPSUH,HES1 expression,and increased Bcl-2 expression(P＜0.05).Compared with the model group,the NICD,RBPSUH and HES1 mRNA and protein expressions significantly decreased after DAPT treatment(P＜0.05),and there was no superposition effect with GLG-ZG.Conclusion GLGZG may play a neuroprotective role by inhibiting inflammatory factors and apoptosis,and inhibiting Notch signaling pathway.
