Proton pump inhibitor pantoprazole promotes colonization of Helicobacter pylori Sydney strain 1 in the mouse stomach
10.3969/j.issn.1671-7856.2025.02.003
- VernacularTitle:质子泵抑制剂泮托拉唑促进幽门螺旋杆菌SS1在小鼠胃内的定植
- Author:
Shuo YAN
1
;
Xue LI
1
;
Chao WANG
1
;
Jiali XU
1
;
Yu CHENG
1
;
Liping ZHANG
1
;
Lei SU
1
;
Jianan GONG
1
Author Information
1. 中国医学科学院医学实验动物研究所,中国医学科学院&北京协和医学院人类疾病比较医学国家重点实验室,国家人类疾病动物模型资源中心,国家动物模型技术创新中心,北京 100021
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
proton pump inhibitor;
Helicobacter pylori infection;
mouse model
- From:
Chinese Journal of Comparative Medicine
2025;35(2):24-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore method for improving the colonization efficiency of Helicobacter pylori(Hp)in the mouse stomach and to determine if the proton pump inhibitor(PPI)pantoprazole can act as a colonization adjuvant to enhance Hp colonization,with the aim of providing an effective tool for establishing an Hp infection mouse model.Methods The Hp Sydney strain 1(SS1)was introduced and solid plate and liquid culture systems were established.The effects of different doses of pantoprazole on gastric acid secretion in mice were compared.The impact of Hp inoculation,alone or combined with pantoprazole pretreatment,on Hp colonization efficiency was analyzed using rapid urease tests,bacterial plate cultures,and TaqMan quantitative polymerase chain reaction.Results PPI pretreatment inhibited gastric acid secretion and promoted Hp colonization in the mouse stomach,to some extent.Conclusions PPI can serve as colonization adjuvants to enhanc e the efficiency of constructing Hp infection mouse models.
