Effect of tetramethylpyrazine on neuroinflammation after cerebral ischemia and hypoxia based on mannose-binding lectin
- VernacularTitle:基于甘露糖结合凝集素探讨川芎嗪对脑缺血/缺氧后神经炎症的影响
- Author:
Yan-zhe DUAN
1
;
Yu-kang SUN
;
Jian-lin HUA
;
Chun-li WEN
;
Hao TIAN
;
Yi YANG
;
Xiu LOU
;
Cun-gen MA
;
Yu-qing YAN
;
Li-juan SONG
Author Information
- Publication Type:Journal Article
- Keywords: ischemic stroke; tetramethylpyrazine; complement system; mannose-binding lectin; inflam-matory factor; neuroinflammation
- From: Chinese Pharmacological Bulletin 2025;41(4):668-676
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of tetrameth-ylpyrazine(TMP)on neuroinflammation after cerebral ischemia and hypoxia via mannose-binding lectin(MBL).Methods Patients diagnosed with ischaemic stroke at Shanxi Provincial People's Hospital were in-cluded in the study,and their clinicopathological data,as well as blood and urine samples,were collected with the consent of the patients and their families.Using these biological samples,differential proteins and tar-gets were identified by proteomic analysis and subse-quently verified with animal experiments.The mice were divided into the sham,dMCAO,and TMP(10,20,40 mg·kg-1)treatment groups.After seven days of drug administration,the modified neurological sever-ity score(mNSS)was used to assess the neurological function.TTC staining was used to detect the volume of cerebral infarction.Motor function was evaluated be-haviourally,and ELISA was used to detect MASP1,sC5b-9,TNF-α,IL-6,and IL-1β.Western blot was used to determine the expression of relevant proteins,such as MBL2,MASP2,and C3.Results Compared with the sham group,the dMCAO group exhibited in-creased neurological impairment,which was signifi-cantly ameliorated by TMP treatment.The expression levels of MBL2,C3 and MASP2 were elevated in the dMCAO group and were reduced following TMP treat-ment.Additionally,the dMCAO group showed elevat-ed expression of inflammatory factors IL-1 β,IL-6 and TNF-α,which were then suppressed by TMP treat-ment.Conclusion TMP inhibits the inflammatory re-sponse after ischemia and hypoxia by regulating MBL,thus attenuating brain injury.
