Construction and comparative study of vaccinia virus VR-1354 infection models in C57BL/6N and BALB/C mice
10.3969/j.issn.1671-7856.2025.02.009
- VernacularTitle:痘苗病毒VR-1354感染C57BL/6N和BALB/C小鼠模型的构建及差异比较研究
- Author:
Yunxiang WU
1
;
Chao WANG
;
Shun LI
;
Xiaohui ZHOU
Author Information
1. 上海师范大学生命科学学院,上海 200233;上海市公共卫生临床中心实验动物部,上海 201508
- Publication Type:Journal Article
- Keywords:
C57BL/6N;
BALB/C;
VR-1354;
infection model;
comparative study
- From:
Chinese Journal of Comparative Medicine
2025;35(2):94-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct mouse models of vaccinia virus infection using C57BL/6N and BALB/C mice infected with vaccinia virus VR-1354(WR(NIH TC-adapted)),and to compare the differences between the models in the two mouse strains.Methods Vaccinia virus VR-1354 was amplified,concentrated,and titrated,according to conventional method.The median lethal doses(LD50)of vaccinia virus V R-1354 for C57BL/6N and BALB/C mice were determined,respectively,and mice from the two strains were infected with the respective LD50.Lung tissues from control and experimental mice were removed for hematoxylin and eosin staining on days 3,7,and 14,and changes in the virus load in the lung tissues were measured at the same times.Results The titer of the amplified vaccinia virus VR-1354 was 3×108 plaque forming units(PFU)/mL.The LD50 values in C57BL/6N and BALB/C mice were 2.5×103and BALB/C 2.8×103PFU,respectively.After infecting mice from both strains with 2.5× 103 PFU of vaccinia virus VR 1354,lung tissue lesions worsened with increasing infection time,but the symptoms of the mice tended to recover after day 7.The virus loads in lung tissues began to increase on day 3 in both strains,peaked on day 7,and then gradually decreased.Conclusions C57BL/6N and BALB/C mouse models infected with vaccinia virus VR-1354 were established successfully.The virus can cause obvious lesions in mouse lung tissues,including inflammatory cell infiltration and alveolar cavity fluid exudation.The degree of disease onset and susceptibility differed between the two mouse strains,with C57BL/6N mice showing higher susceptibility to this virus than BALB/C mice.
