Effects of acteoside on intestinal bacteria and inflammatory factors in mice with ulcerative colitis
- VernacularTitle:毛蕊花糖苷对溃疡性结肠炎小鼠肠道菌和炎症因子的影响
- Author:
Abula AREZIGULI
1
;
Shun-qian FENG
;
Hui-li MA
;
Aimaier ALINUER
;
Li GAO
;
Ming YAN
Author Information
- Publication Type:Journal Article
- Keywords: acteoside; ulcerative colitis; intestinal flora; disease activity index; 16S rRNA; inflammatory factor
- From: Chinese Pharmacological Bulletin 2025;41(4):646-654
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effects of acteoside(ACT)on ulcerative colitis(UC)mice from the per-spective of inflammation and intestinal flora.Methods The UC model was prepared by dextran sulfate sodi-um(DSS)drinking method and treated with ACT for 14 d,during which the activity of the mice was ob-served and the disease activity index(DAI)was scored.ELISA was used to detect the expression of in-flammatory factors.HE staining was used to observe the injury of colon tissue.16S rRNA high-throughput sequencing method was used to detect the structure and abundance of fecal intestinal flora in mice,and the correlation between the flora and inflammatory indica-tors was analyzed.Results After ACT administra-tion,the fear of cold and lazy movement of mice were improved,and the DAI of mice was reduced.The in-tegrity of colon mucosal epithelial cells was restored and mucosal damage was alleviated.The expressions of pro-inflammatory factors MPO,IL-1 β,IL-6,TNF-α,TF and PAF decreased,while the expression of anti-in-flammatory factor IL-10 increased.The abundance of harmful bacteria such as Firmicutes and Bacteroides de-creased,while the abundance of beneficial bacteria such as Bacteroidetes and Alistipes increased.Protective bacteria such as Bacteroidetes and Lachnospira were negatively correlated with IL-1β,IL-6 and other pro-inflammatory factors,while harmful bacteria such as Proteobacteria and Streptococcus were positively correla-ted with the above pro-inflammatory factors.Conclu-sions The therapeutic mechanism of ACT on chronic UC mice is related to its alleviation of inflammation and regulation of intestinal flora,and there is a correlation between inflammatory factors and intestinal flora.
