Effects of high-altitude hypoxia exposure on brain injury in rats based on oxidative stress and aquaporins
10.13699/j.cnki.1001-6821.2025.01.017
- VernacularTitle:基于氧化应激及水通道蛋白探讨高原低氧暴露对大鼠脑损伤的影响
- Author:
Xin-jue ZHANG
1
;
Wang-jie CAO
;
Yun SU
;
Hong-xia GONG
;
Yong HUANG
;
Yong-qi LIU
;
Jian-zheng HE
;
Jia-wang GUO
;
Neng-xian ZHANG
Author Information
1. 甘肃中医药大学 基础医学院,甘肃 兰州 730101;甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,甘肃兰州 730000;敦煌医学与转化教育部重点实验室,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
aquaporins;
high-altitude hypoxia;
high-altitude cerebral edema;
oxidative stress;
blood-brain barrier
- From:
The Chinese Journal of Clinical Pharmacology
2025;41(1):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the brain damage of SD rats under different time points of hypobaric hypoxia exposure.Methods A rat high-altitube cerebral edema(HACE)model was constructed by simulating an altitude of 6 000 m in a hypobaric hypoxia animal experimental chamber.Thirty-six SD male rats were randomly divided into the control group and the hypobaric hypoxia exposure 3,7 and 14 d groups,with 9 rats in each group.Except for the control group,the rats in each group were continuously exposed to hypobaric hypoxia for 3,7,and 14 d.At the end of the modeling period,serum was collected by blood sampling via the abdominal aorta,and brain tissue samples were taken.The wet-to-dry ratio(W/D)of brain tissue was calculated,and the levels of relevant oxidative enzymes in serum and brain tissue were measured.The expression levels of hypoxia-inducible factor-1α(HIF-1α)and aquaporin 4(AQP4)mRNAs in brain tissue were detected by real-time fluorescence quantitative polymerase chain reaction.Results The W/D of brain tissues in the control group and the group exposed to hypobaric hypoxia for 3,7 and 14 d were 4.46±0.12,4.98±0.16,5.07±0.18 and 4.95±0.07;the superoxide dismutase contents were(111.86±2.45),(90.73±1.48),(79.64±2.56)and(55.33±1.45)U·g-1;the glutathione contents were(126.91±5.18),(125.26±1.53),(56.20±2.17)and(122.73±1.78)μg·mL-1;the malondialdehyde contents were(230.94±2.00),(362.65±3.28),(407.34±3.47)and(237.50±1.59)nmol·g-1;the relative expression levels of HIF-1 α mRNA were 1.00±0,2.99±0.49,4.72±0.49 and 1.91±0.28;the relative expression levels of AQP4 mRNA were 1.00±0,2.62±0.34,8.38±0.84 and 5.27±0.42,respectively.Statistically significant differences were found between the above indexes in the 3,7 and 14 d of hypobaric hypoxia exposure group compared with the control group(P＜0.05,P＜0.01).Conclusion Different time of hypobaric hypoxia exposure can up-regulate the expression of AQPs proteins in HACE rats and cause the disruption of the blood-brain barrier,and the HACE model constructed in the hypobaric hypoxia chamber with 6 000 m intervention for 7 d was more stable.
