Effects of Morinda officinalis oligosaccharides exposure during lactation on the Sprague-Dawley maternal rats and their offspring's development
10.3760/cma.j.cn114015-20240202-00076
- VernacularTitle:巴戟天寡糖哺乳期暴露对Sprague-Dawley母鼠及其子代发育影响的研究
- Author:
Manman ZHAO
1
;
Runcheng HE
;
Ying YANG
;
Zeping ZUO
;
Xinyao CAO
;
Chao WANG
;
Nie WEN
;
Sanlong WANG
;
Xingchao GENG
;
Zhibin WANG
;
Xiaobing ZHOU
Author Information
1. 中国食品药品检定研究院，国家药物安全评价监测中心，药物非临床安全评价研究北京市重点实验室，北京 100176
- Publication Type:Journal Article
- Keywords:
Morinda officinalis oligosaccharides;
Lactation;
Animal experimentation;
Development of offspring;
Safety;
Depression, postpartum
- From:
Adverse Drug Reactions Journal
2024;26(9):543-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of maternal exposure to Morinda officinalis oligosaccharides (MOO) during lactation on the Sprague-Dawley (SD) maternal rats and their offspring's growth and development. Methods:Seventy-two female rats with a surviving litter size of ≥ 6 were divided into the excipients control group, MOO low-dose group (50 mg/kg), MOO medium-dose group (160 mg/kg), and MOO high-dose group (500 mg/kg) using a snake-shaped grouping based on body weight, with 18 rats per group. The rats were gavage fed once daily until 20 days of delivery. The response of maternal rats after MOO exposure during lactation, as well as the appearance, response, gross anatomical abnormalities of their F1 and F2 offspring were observed. The body weight and food intake of maternal rats during lactation and those of their offspring before and after weaning were measured. The behavior (central nervous system function) of the F1 and F2 offspring was evaluated using functional observation battery (FOB). The learning and memory function of the F1 offspring was evaluated using Y-maze test. The male and female F1 offspring in the same dose group were mated when they were raised to 10-12 weeks in order to observe the reproductive function of F1 female rats.Results:Compared with the excipients control group, no abnormality was found in the clinical observation of maternal rats in the 3 MOO exposure groups during lactation, and there was no significant differences in their body weight and daily food intake during lactation (all P>0.05). No significant effects were found on the appearance, clinical symptoms, gross anatomy, body weight, and food intake of the F1 and F2 offspring after maternal rats receiving MOO exposure during lactation. In the FOB of the F1 and F2 offspring and the Y-maze test of F1 offspring, few differences in MOO exposure groups were observed and lack of significant dose-response relationship. After pregnancy, there were no statistically significant differences in the number of corpus luteum, implantation number, birth index, delivery index, survival index, and weaning index in F1 female offspring of maternal rats exposed to MOO at different doses during lactation compared with those of the excipients control group (all P>0.05). Conclusions:There were no obvious toxic reactions in maternal rats after exposure to different doses of MOO during lactation, nor in the growth and development, nervous system, learning and memory, and reproductive function of their offspring.
