Optimization of linear calibration using two reference substances under complicated chromatographic condition-taking Cassiae Semen as an example
10.19778/j.chp.2025.03.006
- VernacularTitle:复杂色谱条件下的双标线性校正定位分析方法优化——以决明子饮片为例
- Author:
Lingling JIANG
1
;
Ruiying LIANG
;
Zhechun LI
;
Yi HE
;
Xiao LUO
;
Shuhong WANG
;
Yang YU
;
Chang SU
Author Information
1. 深圳市药品检验研究院(深圳市医疗器械检测中心),国家药品监督管理局中药质量研究与评价重点实验室,广东 深圳 518057
- Publication Type:Journal Article
- Keywords:
Cassiae Semen;
HPLC;
linear calibration using two reference substances;
complicated chromatograph-ic condition;
localization of chromatographic peaks
- From:
Drug Standards of China
2025;26(3):270-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a linear calibration method using two reference substances for seven characteristic peaks of Cassiae Semen under complicated chromatographic condition,and to optimize the method.Methods:Using 15 different types of screened chromatographic columns and 2 components as reference compounds pair,the linear calibration method with 2 reference substances was established to predict the retention time of the other 5 components,and the method was verified by unknown chromatographic columns and unknown samples.Combined with column confirmation number and average coincidence rate of target peaks,the location results were compared comprehen-sively,and the method was optimized according to the defect under the influence of complicated chromatographic condition.Results:The average conformity rate of the target peak of the method before optimization was 73.3％,and the average conformity rate of the target peak of the optimized method was 98.7％.The optimized method has a high-er average peak coincidence rate and a wider range of applicability for the chromatographic column.Conclusion:The optimized linear calibration method using two reference substances can assist the localization analysis of chromato-graphic peaks in the characteristic chromatogram under complicated chromatographic condition.
