Qualitative determination of multiple components in Rhodiola crenulata based on linear calibration with two reference substances assisted HPLC
10.19778/j.chp.2025.03.005
- VernacularTitle:基于双标线性校正法辅助HPLC的红景天多组分定性分析
- Author:
Yang WANG
1
;
Xiaoying GUAN
;
Yaoxuan XIE
;
Junyao LI
;
Bing WANG
;
Xiao LUO
;
Chang SU
;
Shuhong WANG
Author Information
1. 深圳市药品检验研究院(深圳市医疗器械检测中心),国家药品监督管理局中药质量研究与评价重点实验室,广东 深圳 518057
- Publication Type:Journal Article
- Keywords:
Rhodiola crenulata;
HPLC;
linear calibration with two reference substances(LCTRS);
relative reten-tion time(RRT);
substitute reference substance;
chromatographic peak identification
- From:
Drug Standards of China
2025;26(3):263-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a qualitative analysis of Rhodiola crenulata for determing six components inclu-ding gallic acid,salidroside,tyrosol,1,2,3,4,6-O-gallic glucose,rhodiosin,oxorlin-7-O-rhamnoside by HPLC,and to find out the feasibility of the method of linear calibration using two reference substances in qualitative analysis of chromatographic peaks.Methods:The real retention time of 6 components in Rhodiola crenulata on 19 chromatographic columns were determined.Gallic acid and rhodiosin were selected as the reference substances,and the method of linear calibration using these 2 substances was used to predict the retention time.Tyrosol was also chosen as the reference to predict the retention time with the relative retention time method(RRT method).Comparing the accuracy of these two methods.Results:Compared to the RRT method,the method of linear calibration with two reference substances was more accurate for predicting the retention time and more adapatable for many kinds of chromatographic columns.Conclusion:As a new alternative reference substance method,the method of linear calibration using two reference substances can assist chromatographic peak determination better and has broad application prospects.
