Shengmai Yin alleviates myocardial ischemia/reperfusion injury via inhibiting Calpains expression
- VernacularTitle:生脉饮抑制Calpains蛋白表达改善心肌缺血/再灌注损伤的作用及机制
- Author:
Rong MIAO
1
;
Jing-wen GUO
1
;
Ming HUANG
1
;
Hai-shuo REN
1
;
Rui LIU
1
;
Xiao-yu SUN
1
;
Opoku Bonsu FRANCIS
1
;
Qi-long WANG
1
;
Shi-ming FANG
1
;
Ling LENG
1
Author Information
- Publication Type:Journal Article
- Keywords: myocardial ischemia/reperfusion injury; Shengmai Yin; heart function; myocardial injury; Cal-pain; Ca2+
- From: Chinese Pharmacological Bulletin 2025;41(8):1569-1577
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effect of Shengmai Yin on myocardial ischemia/reperfusion in-jury(MI/RI)in vitro and in vivo and to unravel the underlying mechanism.Methods SD rats were divid-ed into the sham group,model group,and Shengmai Yin group(SM).Rat MI/RI model was established.Cardiac function,infarct area,pathological changes,cardiomyocyte apoptosis,macrophage infiltration,and serum cTnT and CK-MB levels were measured.The mRNA and protein expressions of Calpain-1 and Cal-pain-2 were assessed.The hypoxia/reoxygenation(H/R)model was constructed in H9c2 cells.The active ingredients of Shengmai Yin were screened using net-work pharmacology and verified by CCK-8.In the car-diomyocytes H/R model,Fluo-4 AM staining was used to detect the changes of Ca2+levels.Results Com-pared with model group,LVEF and LVFS of Shengmai Yin-treated rats increased,myocardial infarction area was reduced,while myocardial tissue injury was allevi-ated.Myocardial apoptosis rate and the number of macrophages were reduced.Similarly,cTnT and CK-MB levels decreased.In addition,the expression lev-els of Calpain-1 and Calpain-2 mRNA and protein de-creased in the SM treatment group.Under the H/R model,all the active ingredients of Shengmai decoction had protective effects on cardiomyocytes,and the treat-ment could reduce the level of Ca2+in cardiomyocytes.Conclusions Shengmai Yin has protective effects on MI/RI in rats.This effect may be related to the de-crease in Ca2+levels,as well as Calpain-1 and Calap-in-2 mRNA and protein expression.
