Mechanism of let-7b-5p targeting IGF1R to inhibit glioma cell growth
10.13315/j.cnki.cjcep.2025.03.013
- VernacularTitle:let-7b-5p靶向IGF1R调控脑胶质瘤细胞生长的机制
- Author:
Xihong LIU
1
;
Xiaodan DU
1
;
Mengyang FAN
1
;
Liuqing XU
1
;
Liping YANG
1
;
Junlin HOU
1
;
Peiyuan ZHAO
1
Author Information
1. 河南中医药大学中医学院(仲景学院),郑州 450046
- Publication Type:Journal Article
- Keywords:
glioma;
let-7b-5p;
IGF1R;
PI3K/AKT
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(3):359-364
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the expression level of let-7b-5p in glioma and its effects and potential mecha-nisms on U251 cell growth.Methods The expression of let-7b-5p in glioma was detected using qRT-PCR.Data from the CGGA database were analyzed to examine the relationship between the let-7b-5p expression levels,WHO grade and overall survival rates of glioma patients.Transient transfection was used to downregulate the expression of let-7b-5p and IGF1R in U251 cells.The role and potential mechanism of let-7b-5p in the U251 cell were evaluated using qRT-PCR,CCK8 assays,clone formation assays,Western blotting,and double luciferase reporter assays.Results The expres-sion of let-7b-5p in glioma cells(A172:3.64±0.64,V251:4.56±0.52,U87-MG:3.31±0.50)and tissues(2.18±0.22)was significantly higher than that in astrocytes(HMC3:1.00±0.21,P＜0.05 or P＜0.01)and nor-mal brain tissues(1.01±0.19,P＜0.05).Let-7b-5p expression was negatively correlated with WHO grades but pos-itively correlated with survival rates in primary and recurrent glioma patients(P＜0.000 1 and P=0.028,respective-ly).Knockdown of let-7b-5p in U251 cells significantly promoted the growth of glioma cells(CCK8:knockdown group 126.00±12.09 vs miR-NC group 90.93±5.13,P＜0.05)and activated PI3K/AKT signal pathway.Suppressing IGF1R expression in U251 cells reversed the effects of let-7b-5p knockdown on glioma cell growth[CCK8:let-7b-5p knockdown+IGF1R knockdown group(92.08±6.14)vs let-7b-5p knockdown+sh-NC group(116.67.08±8.50)]and PI3K/AKT signal pathway activation.Conclusion Let-7b-5p functions as a tumor suppressor gene in glioma.It may regulate glioma cell growth by targeting IGF1R and modulating PI3K/AKT signal pathway.
