Expression of FAM134B in hepatocellular carcinoma and its clinicopathological significance
10.13315/j.cnki.cjcep.2025.03.007
- VernacularTitle:肝细胞癌中FAM134B表达及其意义
- Author:
Shan HUANG
1
;
Xian WANG
;
Jingjing WEI
;
Zhengsheng WU
Author Information
1. 安徽医科大学基础医学院病理学教研室,合肥 230032;安徽医科大学第二附属医院病理科,合肥 230601
- Publication Type:Journal Article
- Keywords:
hepatocellular neoplasms;
FAM134B;
prognosis;
invasion;
migration;
proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(3):317-323,333
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of FAM134B in hepatocellular carcinoma(HCC)and its clini-cal significance.Methods Immunohistochemical EnVision two-step method was used to detect the expression of FAM134B in 60 cases of HCC tissue and adjacent non-tumor liver tissue.The relationship between FAM134B expres-sion and clinicopathological characteristics and prognosis of HCC was analyzed.Transwell assays,CCK-8 assays and clone formation assays were used to assess the effects of FAM134B knockdown on HCC cell migration,invasion and proliferation.Results FAM134B had a high expression rates of 70.0％(42/60)in HCC tissues and 40.0％(24/60)in adjacent normal tissues.Compared to adjacent non-tumor tissues,FAM134B expression was significantly higher in HCC tissues(P all＜0.001),and it was positively correlated with vascular invasion,tumor maximum diameter,and early recurrence(P all＜0.05).Survival analysis showed that high expression of FAM134B was an independent poor prognostic factor and a high recurrence risk factor for HCC patients(P all＜0.05).In vitro experiments demonstrated that knockdown FAM134B inhibited HCC cell migration,invasion and proliferation(P all＜0.05).GSEA analysis u-sing the TCGA database indicated that the expression of FAM134B was positively correlatied with the AKT/mTOR path-way.Conclusion High expression of FAM134B may be a marker of high recurrence risk and poor prognosis in HCC and holds potential as a therapeutic target for HCC.
