Expression of SNAP23 in esophageal squamous cell carcinoma tissue and its effect on invasion of esophageal cancer cells
10.13315/j.cnki.cjcep.2025.03.004
- VernacularTitle:食管鳞状细胞癌组织中SNAP23的表达及其对食管癌细胞侵袭的影响
- Author:
Jiao CHEN
1
;
Qing LIU
;
Shutao ZHENG
;
Huifang LI
;
Wei WANG
;
Tao LIU
;
Conggai HUANG
;
Tianyuan PENG
;
Xiaomei LU
Author Information
1. 新疆医科大学第一附属医院临床医学研究院/省部共建中亚高发病成因与防治国家重点实验室,乌鲁木齐 830011
- Publication Type:Journal Article
- Keywords:
esophageal neoplasms;
squamous cell carcinoma;
SNAP23;
invasion and metastasis
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(3):298-303
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To analyze the expression of SNAP23 in esophageal squamous cell carcinoma(ESCC)and its influence on invasion of ESCC cells.Methods A total of 41 cases of ESCC and paired normal adjacent tissue(NAT)were collected.The expression and localization of SNAP23 were detected by immunohistochemical EnVision method.The differences of SNAP23 expression levels between ESCC tissues and NAT tissues were compared to analyze the relationship between SNAP23 expression and clinicopathological characteristics.The expression level of SNAP23 in human immortalized esophageal epithelial cells SHEE and ESCC cell lines TE-1,TE-13 and KYSE150 were detected by Western blot.Lentiviral vector transfection technique was used to construct stable transfection cell lines with knock-down and overexpression of SNAP23 gene.The effect of SNAP23 on invasion of ESCC cell line TE-13 was evaluated by cell invasion experiment.Results The expression of SNAP23 in ESCC was significantly higher(53.7％,22/41)than that in the NAT group(19.5％,8/41),the difference was statistically significant(x2=10.303,P＜0.01).The expression level of SNAP23 in ESCC tissues was significantly correlated with maximum tumor diameter(x2=4.193,P＜0.05)and invasion depth(x2=7.264,P＜0.05),but was not correlated with patient gender,age,tumor site,tumor type,pathologic grade,vascular embolus,nerve invasion and lymph node metastasis(P＞0.05).Compared with human immortalized esophageal epithelial cells SHEE,SNAP23 was highly expressed in ESCC cell lines(P＜0.000 1).Compared with the sh-NT group,the invasion of ESCC cell line TE-13 was inhibited when SNAP23 expression was knocked down(P＜0.000 1);compared with the Vector group,the invasion of ESCC cell line TE-13 was enhanced after overexpression of SNAP23(P＜0.000 1).Conclusion SNAP23 is highly expressed in ESCC tis-sue and cell lines,and its expression level in ESCC tissues is positively correlated with tumor maximum diameter and invasion depth;SNAP23 promotes the invasion of ESCC cells.
