Qingluo Yin inhibits synovial angiogenesis induced by adjuvant in ar-thritis rats by regulating HIF-1α/VEGF pathway
10.12092/j.issn.1009-2501.2025.03.009
- VernacularTitle:清络饮通过调控HIF-1α/VEGF通路抑制佐剂诱导关节炎大鼠的滑膜血管新生
- Author:
Peipei WANG
1
;
Linkun PAN
;
Kui YANG
;
Dandan FENG
Author Information
1. 皖南医学院弋矶山医院,芜湖 241001,安徽
- Publication Type:Journal Article
- Keywords:
Qing-Luo-Yin(QLY);
rheumatoid ar-thritis;
Traditional Chinese Medicine(TCM);
Bi Zeng;
synovitis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(3):366-373
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the involvement of HIF-1α/VEGF regulation in its anti-angiogenesis effects using adjuvant-induced arthritis(AIA)rats.METHODS:AIA rats were orally treated by QLY ex-tract for 24 days.After sacrifice,the joints were subjected to histological examination,while the blood was used in ELISA or biochemical tests.In ad-dition,HUVEC cells were treated by QLY in vitro.MTT,wound-healing and tube-formation experi-ments were then performed.Expression of some relevant proteins in cells were investigated.RE-SULTS:Compared to the healthy controls,obvious synovial invasion and angiogenesis occurred in AIA rats.Blood levels of HIF-1α,VEGF,PDGF,and TGF-β1 were increased,while the ratio of MDA/SOD was decreased a lot.After QLY treatment,all these ab-normalities were attenuated.In vitro experiments,QLY showed notable potentials in inhibiting prolifer-ation,migration and tube-constructing abilities of HUVEC cells.Furthermore,it suppressed the ex-pression of p-MEK/MEK and p-ERK/ERK.CONCLU-SION:QLY can reduce the pathological functions of vascular endothelial cells by inhibiting HIF-1α/VEGF,and it consequently eased AIA-related abnor-mal angiogenesis in AIA rats'joints.
