Study on the efficacy and mechanism of Qingre xiaoyanning against influenza A H3N2 virus
10.12092/j.issn.1009-2501.2025.03.007
- VernacularTitle:清热消炎宁抗甲型流感H3N2病毒的药效及作用机制研究
- Author:
Shasha ZHOU
1
;
Xueqing CHENG
;
Dongdong PENG
;
Xiaoqing WANG
;
Lijun FU
;
Wenxi XIAO
;
Guomin ZHANG
Author Information
1. 湖南中医药大学,中西医结合学院,长沙 410208,湖南;天地恒一制药股份有限公司,药物研究院,长沙 410331,湖南
- Publication Type:Journal Article
- Keywords:
Qingre xiaoyanning;
influenza a;
cyto-kines;
immunomodulatory effect
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(3):347-354
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the antiviral effica-cy and mechanism of Qingre Xiaoyanning(QRXYN)in vivo,and provide experimental basis for their prevention and treatment of influenza A virus.METHODS:We constructed a mouse model infect-ed with influenza A H3N2 virus.To evaluate the therapeutic effect of QRXYN on influenza A virus,we measured the body weight changes,pathologi-cal changes in lung tissue,hemagglutination titer,and viral load in mouse.To evaluate the possible mechanism of QRXYN's anti influenza A virus infec-tion,we used the ELISA to measure the levels of TNF-α,IL-1β,IL-4,IFN-γ,and vascular cell adhesion molecule-1(VCAM-1)in mouse bronchoalveolar Ia-vage fluid;used flow cytometry to assess the pro-portions of macrophages(F4/80),helper T lympho-cytes(CD4+T lymphocytes),and natural killer(NK)cells in lung tissue;and used Western blotting to detect the expression of Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MYD88),inhibitor of kappa B kinase-β(IKK-β),NF-kappa-B inhibitor al-pha(IκBα),and phospho-IKB alpha(p-IκBα)in lung tissue.RESULTS:Compared to the model group,both Oseltamivir and QRXYN can alleviate the se-verity of lung tissue lesions in mice,decrease the blood coagulation titer and viral load of mouse lung tissue(P＜0.01),lower the levels of TNF-α,IL-4,and VCAM-1 in bronchoalveolar lavage fluid(P＜0.05,P＜0.01),reduce the proportion of macro-phages(P＜0.05,P＜0.01),and increase the propor-tion of CD4+T lymphocytes and NK cells(P＜0.05,P＜0.01).Additionally,oseltamivir can reduce the ex-pression of MYD88 protein in mouse lungs(P＜0.05),while QRXYN can decrease the expression of IKK-β and P-IκBα proteins in mouse lungs(P＜0.05).CONCLUSION:QRXYN have good in vivo antiviral ef-fects against the influenza A virus,and their mecha-nism may be related to the regulation of the immu-nologic function and NF-κB signal pathway.
