Antiviral mechanism of Euphorbia helioscopia diterpenoids against Zika virus in vitro
- VernacularTitle:泽漆二萜化合物体外抗寨卡病毒机制
- Author:
Pan-pan PANG
1
;
Xiong QIU
;
Ying-jie JIANG
;
Xin-yue LIU
;
Wei-zhe MA
;
Jian-qiu-rong YIN
;
Wei-lie XIAO
;
Chang-bo ZHENG
Author Information
- Publication Type:Journal Article
- Keywords: Zika virus; Euphorbia helioscopia diterpe-noid compound; viral entry inhibition; prM protein; molecular docking; natural product antivirals
- From: Chinese Pharmacological Bulletin 2025;41(8):1436-1444
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the anti-Zika virus(ZIKV)mechanism of diterpenoid compound 9 from Euphorbia helioscopia in vitro.Methods The cytotox-icity of compound 9 was evaluated using the CCK-8 as-say.A ZIKV-infected Vero cell model was established,and the antiviral activity was assessed through RT-qPCR,plaque assay,Western blot,and immunofluores-cence.Furthermore,the mechanism of action was elu-cidated using multi-cell line validation,nanoparticle tracking analysis,cellular thermal shift assay,and mo-lecular docking.Results In Vero cells,compound 9 exhibited an EC50 of(3.95±0.15)μmol·L-1 and a CC50 of(272.12±8.56)μmol·L-1,demonstrating significantly higher antiviral efficacy than the positive control drug ribavirin(RBV).Its virus inactivation effect was time-dependent and could significantly re-duce viral load and plaque formation.Studies revealed that compound 9 altered the physicochemical properties of ZIKV particles,including reducing surface charge and increasing particle size distribution.Additionally,it significantly enhanced the thermal stability of the prM protein.Molecular docking analysis indicated that compound 9 formed a high-affinity interaction with the prM protein(binding energy:-38.52 kJ·mol-1)and stabilized its structure through hydrophobic interac-tions.Conclusion Compound 9 exerts in vitro anti-ZIKV activity by directly inactivating the virus,disrup-ting viral particle integrity,and targeting the prM pro-tein.
