Fructus Akebiae induces apoptosis via regulating ROS-mediated PI3K-Akt signaling pathway in non-small cell lung cancer cells
10.12092/j.issn.1009-2501.2025.03.006
- VernacularTitle:预知子经ROS介导的PI3K-Akt通路诱导非小细胞肺癌细胞凋亡
- Author:
Wanli GAO
1
;
Qichun ZHOU
1
;
Sumei WANG
1
Author Information
1. 广州中医药大学第二临床医学院 中医药防治非小细胞肺癌临床与基础研究团队,广州 510520,广东
- Publication Type:Journal Article
- Keywords:
Fructus Akebiae;
non-small cell lung cancer;
apoptosis;
PI3K-Akt signaling pathway;
ROS
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(3):339-346
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the way and mecha-nism of cell death induced by Fructus Akebiae in non-small cell lung cancer(NSCLC)cells.METH-ODS:CCK-8,Hochest33342/PI staining,and colony formation experiments were used to detect cell via-bility and proliferation.Western blot was used to detect the expression of cell death-related proteins ATG5,HMGB1,GPX4,apoptosis-related proteins,and PI3K-Akt signaling pathway-related proteins.Flow cytometry was used to detect the apoptosis rate.DCFH-DA fluorescent probes were used to de-tect the levels of total intracellular ROS under a flu-orescence microscope.RESULTS:Fructus Akebiae significantly inhibited the cell viability and prolifera-tion of human NSCLC cells(P＜0.05).The expression of apoptosis-related proteins BAX,Cleaved cas-pase3,and Cleaved caspase9 in NSCLC cells was sig-nificantly increased(P＜0.05),while p-PI3K,p-Akt,and BCL2 were markedly decreased(P＜0.05).Fruc-tus Akebiae was found to clearly increase the apop-tosis rate and the levels of total intracellular ROS(P＜0.05).The antioxidant NAC significantly reversed apoptosis,ROS production and regulation of PI3K-Akt pathway related proteins induced by Fructus Akebiae(P＜0.05).CONCLUSION:Fructus Akebiae in-duces apoptosis via regulating ROS-mediated PI3K-Akt signaling pathway in non-small cell lung cancer cells.
