Network pharmacological analysis of berberine inhibiting breast can-cer cell proliferation and in vitro cell validation
10.12092/j.issn.1009-2501.2025.03.005
- VernacularTitle:小檗碱抑制乳腺癌细胞增殖的网络药理学分析及体外细胞验证
- Author:
Huihui ZHANG
1
;
Le JIN
;
Su LIU
;
Hongxiao CHEN
;
Zhaolin CHEN
;
Liqin TANG
Author Information
1. 安徽医科大学临床药理研究所,抗炎免疫药物教育部重点实验室,合肥 230032,安徽
- Publication Type:Journal Article
- Keywords:
network pharmacology;
berberine;
breast cancer;
AKT1
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(3):332-338
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the mechanism of berberine on breast cancer cells based on network pharmacology and in vitro cell experiments.METH-ODS:Firstly,berberine and breast cancer were tak-en as the research objects,the intersection targets of the two were screened by VEEN diagram,GO function and KEGG enrichment analysis were per-formed by R language,and molecular docking and visualization were carried out by Autodock Vina and Pymol software.Then,berberine treated breast cancer MCF-7 cells for 24 h,and then in vi-tro cell experiments were performed.CCK-8 was used to detect cell viability,Edu and plate cloning were used to detect cell proliferation and cloning,and apoptosis was detected by An-nexin V-FITC/PI double staining and Western blot.Laser confocal and CETSA were used to verify the binding effect of berberine and AKT1 protein.RESULTS:The results of network pharmacology showed that berberine had a good binding to the core targets AKT1,AKT2 and MAPK3.Berberine(20,40,80 μmol/L)signifi-cantly inhibited the proliferation and cloning ability of MCF-7 cells in a concentration-dependent man-ner(P＜0.05,P＜0.01).The results of laser confocal and CETSA experiments showed that berberine and AKT1 had a binding effect,and the stability of the two was enhanced after the combination.CONCLU-SION:Berberine inhibits MCF-7 cell proliferation and induces apoptosis in human breast cancer cells by targeting binding to AKT1 protein.
