Effect of sodium butyrate on development of in vitro 3D cultured mouse preantral follicles
10.16303/j.cnki.1005-4545.2025.06.26
- VernacularTitle:丁酸钠对体外3D培养的小鼠腔前卵泡发育的影响
- Author:
Xiaohuan LIU
1
;
Jiayi YU
;
Qi LIU
;
Xianyu XU
;
Yuguo YUAN
;
Fenglei CHEN
Author Information
1. 扬州大学兽医学院,江苏扬州 225009
- Publication Type:Journal Article
- Keywords:
3D culture;
preantral follicle;
sodium butyrate;
oocyte
- From:
Chinese Journal of Veterinary Science
2025;45(6):1305-1313
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to evaluate the effects of sodium butyrate(NaBu)on preliminary follicle development of mice,using an in vitro 3D culture system.Preantral follicles were isolated from mouse ovaries(12.5 days post-birth)and cultured in vitro,NaBu was added for treatment during the culture process.Follicle morphology was observed and follicular diameter,survival rate,antral formation rate,oocyte maturation rate and early embryonic development were evaluated.The re-sults showed that an in vitro 3D culture system for preantral follicles of mice was successfully es-tablished.Matured follicles were cultured following an initial induction period of 8 days,after which M Ⅱ-stage oocytes were effectively expelled.During the process of follicle culture,the addition of varying doses(0.05-1.00 mmol/L)of NaBu from 0th day to 8 days had not showed any significant promotion in follicle development at any of the tested concentrations.NaBu at a dose of 0.10 mmol/L significantly increased antral formation rate(treated on 4th day for 2 days).In conclusion,short-term administration of appropriate doses of NaBu could significantly facilitate in vitro preantral follicle development and oocyte maturation by ensuring sustained developmental potential within follicles.This study may provide a theoretical basis for sodium butyrate to improve the reproduc-tive capacity of livestock and poultry in the breeding process.
