Vitamin D3 on neutrophil calcium uptake and LC3 autophagy function in hypocal-cemic dairy cows
10.16303/j.cnki.1005-4545.2025.06.21
- VernacularTitle:VD3对低钙血症奶牛中性粒细胞钙吸收和LC3自噬功能的影响
- Author:
Xinquan LYU
1
;
Siyao LI
;
Bingbing ZHANG
;
Chuang XU
;
Wei YANG
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319;四川农业大学动物医学院,四川成都 611100
- Publication Type:Journal Article
- Keywords:
Ca2+;
neutrophil;
subclinical hypocalcemia;
LC3;
VD3
- From:
Chinese Journal of Veterinary Science
2025;45(6):1268-1272
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of VD3 on the concentration of Ca2+in neutrophils of subclinical hypocalcemia dairy cows and the autophagy of LC3,peripheral blood of dairy cows was collected and neutrophils were isolated and extracted.Western blot was used to detect the abundance of LC3 autophagy pathway-related proteins(LC3-Ⅱ,P62,ATG5)in cells.The intracellular Ca2+concen-tration of neutrophils was detected by flow cytometry.The LC3 autophagosome was detected by la-ser confocal technology.By adding VD3 to affect intracellular Ca2+concentration levels,the effects of vitamin D3 on calcium absorption and LC3 autophagy function were investigated.Western blot result showed that the protein abundances of LC3 and ATG5 in neutrophils of subclinical hypocal-cemia dairy cows decreased,while the protein abundance of p62 increased,indicating that the auto-phagy function of LC3 in subclinical hypocalcemia dairy cows was weakened.After VD3 treatment,the flow cytometry results showed that VD3 promoted the increase of Ca2+concentration levels in neutrophils.The Western blot results showed that the LC3 autophagy function of neutrophils from healthy cows and subclinical hypocalcemia cows was enhanced under the action of VD3,indicating that VD3 promoted the concentration of Ca2+in neutrophils and then affected the LC3 autophagy function.
