Experimental study of the ultraviolet A-riboflavin cross-linking corneal stro-mal lenticule punctal plug for the treatment of dry eyes in rabbits
10.13389/j.cnki.rao.2025.0051
- VernacularTitle:紫外线A-核黄素交联后的角膜基质透镜泪道栓治疗兔干眼的实验研究
- Author:
Fan YANG
1
;
Fang TIAN
;
Cheng PEI
;
Juan LI
;
Yong LI
;
Yi SHAO
Author Information
1. 710000 陕西省西安市,西安交通大学附属第一医院;710000 陕西省西安市,西安市人民医院(西安市第四医院)眼科,陕西省眼科医院
- Publication Type:Journal Article
- Keywords:
ultraviolet A-riboflavin cross-linking;
corneal stromal lenticule punctal plug;
dry eyes;
tear film function
- From:
Recent Advances in Ophthalmology
2025;45(4):286-290
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of the ultraviolet A-riboflavin cross-linking corneal stromal lenticule punctal plug for the treatment of dry eyes in rabbits.Methods Thirty-two New Zealand white rabbits(64 eyes)were se-lected and randomly divided into a normal group,a model group,a conventional plug group and a cross-linking plug group,with 8 rabbits(16 eyes)in each group.Rabbits in the normal group were not treated.In the model group,moderate-to-se-vere dry eye rabbit models were constructed but no treatment was given.Rabbits in the conventional and cross-linking plug groups were implanted with conventional and cross-linking plugs after model construction,respectively.The tear film func-tions of the experimental rabbits,including tear secretion volume(SIT),corneal fluorescein sodium staining,tear film break-up time(BUT),and tear meniscus height(TMH),were detected before the intervention,1 day,1 week,2 weeks,and 4 weeks after the intervention.In addition,the corneas of each group were collected for hematoxylin and eosin(HE)staining to observe the changes of the corneal epithelium 4 weeks after the intervention.Finally,the non-cross-linking and cross-linking corneal stromal lenticules were placed separately in 1 g·L-1 of type Ⅰ collagenase to observe the change of the lenticule diameter over time.Results In the cross-linking plug group,the SIT,BUT and TMH values 2 and 4 weeks after the intervention were significantly higher than those before the intervention(all P＜0.01).In the conventional plug group,the SIT,BUT and TMH values 4 weeks after intervention were significantly higher than those before the intervention(all P＜0.01).After 4 weeks of intervention,the corneal staining of rabbits in the cross-linking plug group was better than that in the conventional plug group and model group.The HE staining results showed that in the cross-linking plug group,the corneal epithelial thickness of rabbits was basically normal,epithelial cells were basically arranged in order,and a single layer of columnar epithelial cells was observed on the basal layer.The dissolution time of the corneal stromal lenticule after cross-linking was significantly increased,compared with that of the non-cross-linking one.Conclusion The ultraviolet A-riboflavin cross-linking corneal stromal lenticule punctal plug has a more stable therapeutic effect on the rabbit dry-eye model than the conventional one,so it is expected to provide a novel approach for the clinical treatment of dry eyes.
