Effects of Dendrimer-N-acetylcysteine on retinal revascularization in oxygen-induced retinopathy mice and related mechanisms
10.13389/j.cnki.rao.2025.0049
- VernacularTitle:树枝状大分子N-乙酰半胱氨酸(D-NAC)对氧诱导视网膜病变小鼠视网膜血管重建的影响及其作用机制
- Author:
Jing YANG
1
;
Baoqi HU
;
Rujia LIU
;
Yun QI
Author Information
1. 710061 陕西省西安市,西安交通大学第一附属医院健康医学科
- Publication Type:Journal Article
- Keywords:
Dendrimer;
N-acetylcysteine;
oxygen-induced retinopathy;
revascularization;
inflammation
- From:
Recent Advances in Ophthalmology
2025;45(4):276-280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Dendrimer-N-acetylcysteine(D-NAC)on retinal revascularization in oxygen-induced retinopathy(OIR)mice and related mechanisms.Methods Fourteen healthy C57BL/6J mice aged 7 days were used to establish an OIR mouse model.The mice were then divided into a control group and an experimental group.The control group was injected intravitreally with 1 μL of Dendrimer at a concentration of 1 g·L-1 into the left eye.The experimental group was injected intravitreally with 1 μL of D-NAC at a concentration of 1 g·L-1 into the right eye.Fluo-rescence staining of retina flat-mounting and Photoshop software were used to calculate the areas of vaso-obliteration and pathological neovascularization in each group of mice.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the mRNA expression levels of inflammatory factors in the mouse retina.Enzyme-linked immu-nosorbent assay(ELISA)was used to detect the protein expression of tumor necrosis factor-α(TNF-α)in the mouse reti-na.SPSS18.0 software was used for statistical analysis of data.Results The area of vaso-obliteration in the experimental group[(13.55±3.42)mm2]was 38.18％smaller than that in the control group on the 17th day after birth[(21.92±3.13)mm2](t=17.76,P＜0.01).The area of pathological neovascularization in the experimental group[(7.24±2.11)mum2]was significantly smaller than that in the control group[(10.12±2.74)mm2](t=2.91,P＜0.05).The relative mRNA ex-pression levels of TNF-α,interleukin-1 β(IL-1β),interleukin-6(IL-6),intercellular adhesion molecule 1(ICAM-1)and c-c motif chemokine ligand-7(CCL-7)in the mouse retina of the experimental group were significantly lower than those of the control group on the 16th day after birth(all P＜0.05).The relative mRNA expression levels of the astrocyte activation marker,glial fibrillary acidic proteins(GFAP),and the microglial activation marker,ionized calcium binding adaptor mole-cule 1(IBA-1)in the mouse retina of the experimental group were significantly lower than those of the control group on the 16th day after birth(both P＜0.05).ELISA results showed that the expression of TNF-α proteins in the mouse retina of the experimental group[(16.40±5.58)ng·g-1]was 25.49％lower than that of the control group[(22.01±5.82)ng·g-1]on the 16th day after birth(t=7.81,P=0.002).Conclusion Intravitreal injection of D-NAC may promote retinal revas-cularization in OIR mice by reducing levels of retinal inflammatory factors and inhibiting the activation of astrocytes and mi-croglial cells.
