Mechanism of Weissella viridescens induces trained immunity
10.16303/j.cnki.1005-4545.2025.06.06
- VernacularTitle:绿色魏斯菌诱导驯化免疫及其作用机制
- Author:
Xin AN
1
;
Chengkai ZHOU
1
;
Liang ZHANG
1
;
Zhenzhen LIU
1
Author Information
1. 吉林大学动物医学学院,长春 130062
- Publication Type:Journal Article
- Keywords:
W.viridescens;
trained immunity;
macrophages(PMs);
AKT/mTOR/HIF-1α
- From:
Chinese Journal of Veterinary Science
2025;45(6):1143-1149
- CountryChina
- Language:Chinese
-
Abstract:
Weissella viridescens(W.viridescens)was used as the trained immunity inducer.We mo-nitored the protective efficacy against lethal infection of Candida albicans.After W.viridescens training,inflammatory mediator level was measured by NO assay kit upon re-stimulation,and the bacterial killing ability of peritoneal macrophages(PMs)was detected by counting CFU after Staphylococcus aureus(S.aureus)infection.The expression proteins in the classical signaling pathway of trained immunity were detected.Meanwhile,the PMs were pretreated with the corre-sponding inhibitors for the corroborative mechanism.The results showed that W.viridescens could induce trained immunity.The survival rate of Galleria mellonella treated with W.viridescens was still as high as 70％at 48 h,which protected against lethal infection of Candida albicans.W.viri-descens-trained PMs presented immune-enhancing activity,producing more inflammatory mediator(NO)after LPS stimulation and accelerating the clearance of S.aureus.To our delight,the high ex-pression levels of AKT/mTOR/HIF1α were observed under W.viridescens training.Moreover,in-hibiting the AKT/mTOR/HIF1α signaling with inhibitors dampened the trained response to LPS after W.viridescens training.Altogether,these results suggested that W.viridescens induces trained immunity through the AKT-mTOR-HIF-1α pathway to enhance the pro-inflammatory and anti-in-fective capacities of host cells.
