Establishment of UPLC-ELSD Fingerprint and Determination of 9 Components of Xuemai Shutong Granules
10.3870/j.issn.1004-0781.2025.04.023
- VernacularTitle:血脉疏通颗粒UPLC-ELSD指纹图谱的建立及9种成分含量测定
- Author:
Sumei LI
1
;
Xiaoli BI
1
;
Weitao CHEN
1
;
Aili XU
1
;
Guanlin XIAO
1
;
Jingnian ZHANG
1
;
Jieyi JIANG
1
;
Yangxue LI
1
Author Information
1. 广东省第二中医院(广东省中医药工程技术研究院)/广东省中医药研究开发重点实验室,广州 510095
- Publication Type:Journal Article
- Keywords:
Xuemai Shutong granules;
Ultra high-performance liquid chromatography-evaporative light scattering detector;
Fingerprint;
Content determination
- From:
Herald of Medicine
2025;44(4):648-655
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprint of Xuemai Shutong granules by UPLC-ELSD and determine the contents of 9 components in the preparation simultaneously.Methods The UPLC-ELSD was used to establish the fingerprint of Xuemai Shutong granules,and determine the content of its 9 components.The similarity evaluation system,systematic,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used to evaluate the quality of different batches of preparation.Results The similarity degrees of UPLC-ELSD fingerprints of 11 batches of Xuemai Shutong granules were from 0.929 to 0.978,17 common peaks were calibrated,of which 11 peaks were identified:peak 3(notoginsenoside R1),peak 4[ginsenoside Rg,(Re)],peak 5(notoginsenoside R2),peak 6(ginsenoside Rb,),peak 9(astragaloside Ⅳ),peak 10(ginsenoside Rk3),peak 11(ginsenoside Rh4),peak 12[20(S)-ginsenoside Rg3],peak 13[20(R)-ginsenoside Rg3],peak 14(ginsenoside Rk1),peak 15(ginsenoside Rg5).The stoichiometric analysis divided 11 batches of samples into 2 classes,and the 2 principal components in PCA analysis reflected the information of 17common peaks,10 peaks which affected the quality difference are screened out.The linear relationship of the 9 components was good in their respective quality ranges in the content analysis(r＞0.999 2),the average recovery rate were between 95.02％-97.78％and the RSD were 0.69％-1.70％(n=6).The contents of notoginsenoside R1,notoginsenoside R2,ginsenoside Rb1,astragaloside Ⅳ,ginsenoside Rh4,20(S)-ginsenoside Rg3,20(R)-ginsenoside Rg3,ginsenoside Rk1 ginsenoside Rg5 in the 11 batches of Xuemai Shutong granules were 0.087 5-0.187 6,0.494 3-0.688 6,0.448 1-0.705 5,0.192 2-0.270 8,1.492 5-2.077 6,0.316 0-0.463 8,0.254 5-0.382 0,0.117 6-0.163 9,3.407 7-4.706 4 mg·g-1,respectively.Conclusions The established fingerprint and content determination method was accurate and reliable,which can improve the quality standard of Xuemai Shutong granules,and provide reference for its overall quality evaluation.
