Sequence analysis of a novel allele HLA-A*11:193 and its encoding three-dimensional space of protein molecules
10.13431/j.cnki.immunol.j.20250043
- VernacularTitle:HLA新等位基因HLA-A*11:193序列分析及蛋白质三维空间结构模拟
- Author:
Yan LIU
1
;
Qi ZUO
1
;
Jingru SHAO
1
;
Xiangmin NIE
1
;
Peicong ZHAI
1
Author Information
1. 250000 济南,山东省血液中心
- Publication Type:Journal Article
- Keywords:
Human leukocyte antigen;
Sequencing typing;
New allele;
Three-dimensional structure
- From:
Immunological Journal
2025;41(4):285-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the sequence variation of human leukocyte antigen(HLA)novel allele A11:193 and to simulate the three-dimensional structure of the protein molecule.Methods A sample with abnormal allele results was found by PCR-SBT sequencing and identified by single allele specific sequencing.The 3D structure of the encoded protein was analyzed by Swiss-Model.Results Compared with HLA-A*11:01:01,which has the highest homology,exon 4 nt 662 of this sample has a base substitution of A→G,and its corresponding codon 197 is changed from CAT to CGT,which is changed from histidine(His)to arginine(Arg).Conclusion A new allele of HLA-A was confirmed.The allele sequence was named HLA-A11:193 by the WHO HLA Factor Nomenclature Committee and the three-dimensional structure of the protein molecule encoded by HLA-A11:193 was simulated.There was no significant difference in the three-dimensional structure of the encoded protein between it and HLA-A*11:01:01.
