CRISPR/Cas9 Technology:Exploring The Functions of lncRNAs and Their Roles in Cancer Progression
10.13865/j.cnki.cjbmb.2025.01.1194
- VernacularTitle:CRISPR/Cas9技术:探索lncRNA的自身功能以及在癌症进程中的作用
- Author:
Xin LI
1
;
Ying HU
1
;
Yu-Ming WANG
1
Author Information
1. 昆明医科大学第二附属医院检验科,昆明 650000
- Publication Type:Journal Article
- Keywords:
clustered regularly interspaced short palindromic repeats and CRISPR-related protein 9(CRIAPR/Cas9);
long non-coding RNA(lncRNA);
gene regulation;
cancer
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(3):364-375
- CountryChina
- Language:Chinese
-
Abstract:
The emergence of CRISPR/Cas system has greatly promoted the progress in the field of gene editing,especially CRISPR/Cas9 system,which has become a core tool in biomedical research.Long noncoding RNAs(lncRNAs)play a key role in gene regulation,cell differentiation and the development of a variety of diseases.Especially in cancer research,lncRNAs have important application prospects as cancer biomarkers and therapeutic targets.However,lncRNAs are generally characterized by low abun-dance and poor conservation,which limits the study of their functions by traditional means.CRISPR/Cas9 technology provides an efficient,flexible and accurate tool for lncRNA research,which significantly accelerates the progress in this field.This paper first reviews the basic principles of CRISPR/Cas9 system and its wide applications in gene editing,including CRISPR knockout,knock-in,interference,activation and other functional systems.These technologies can not only screen key lncRNAs in specific biological processes,but also be used for gene function research to explore their roles in diseases.This article focu-ses on the analysis of CRISPR/Cas9 technology in the study of lncRNA functions,regulatory mecha-nisms,and its key applications in tumor research.In addition,the article also summarizes the methods of genome-wide screening by CRISPR/Cas9 to identify functional lncRNAs,and discusses the roles of these lncRNAs in cancer cell proliferation,migration,invasion and drug resistance.CRISPR/Cas9 knockout system can efficiently knock down lncRNA genes and reveal their specific functions in gene regulation.At the same time,CRISPR activation and interference technology provide a new idea for the research of non-coding genes,and further explore its clinical application in cancer and other diseases by regulating the expression level of lncRNAs.The article also discusses the potential of CRISPR technology in future ln-cRNA research,especially the progress in solving technical problems such as genome complexity,targe-ting efficiency and off-target effects.As mentioned in the review,CRISPR/Cas9 technology not only pro-vides a powerful tool for studying lncRNAs,but also provides new ideas and opportunities for developing new means of cancer diagnosis and treatment in the future.
