Study on the sequential promotion of angiogenesis by poly(lactic-co-glycolic acid)microcapsules encapsulating vascular endo-thelial growth factor A
10.13591/j.cnki.kqyx.2025.06.002
- VernacularTitle:包封血管内皮生长因子A的聚乳酸-聚乙醇酸共聚物微囊序贯促进血管生成效果的研究
- Author:
Lihong YUAN
1
;
Ying WANG
;
Jiteng LIU
;
Ruizhen LIANG
;
You WU
Author Information
1. 南京医科大学附属口腔医院第七门诊部,江苏南京(210029);口腔疾病研究与防治国家级重点实验室培育建设点,江苏南京(210029);江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Publication Type:Journal Article
- Keywords:
poly(lactic-co-glycolic acid)microcapsules;
vascular endothelial growth factor A;
controlled release;
angiogenesis
- From:
STOMATOLOGY
2025;45(6):406-411,417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To control the stepwise release of vascular endothelial growth factor A(VEGF-A)within the microcapsules,and to analyze the effects of the microcapsules on cellular angiogenic capability.Methods VEGF-A encapsulated poly(lactic-co-gly-colic acid)(PLGA)microcapsules were prepared using a method combining dual-channel coaxial injection and continuous flow technol-ogy.The release and degradation performance of the microcapsules were characterized using a phosphate-buffered saline(PBS)soaking method.The biocompatibility of the microcapsules was assessed through the CCK-8 method and Calcein-AM/PI staining method.The impact of microcapsule extract on cellular angiogenesis ability was examined by conducting cell scratch assays and tubule formation ex-periments.Results The microcapsules were round in shape,with their particle diameter measuring in the range of hundreds of mi-crometers.Microcapsules with a molecular weight(Mw)-12 ku can release a large amount of VEGF-A in the initial phase,while Mw-30 ku ones had the capacity to provide a stable,long-term,low-dose release of VEGF-A.Microcapsules of Mw-12 ku exhibited outstanding potential for enhancing the healing of cell scratch wounds in the initial phase.Moreover,within the 0-12 day period,the two types of microcapsule extracts significantly enhanced the ability of cells to form tubules in vitro.Conclusion This study successfully regulated the release profile of VEGF-A by adjusting the molecular weight of PLGA,achieving an initial rapid and substantial release of VEGF-A followed by a sustained slow release over time,while maintaining its biological activity throughout the process.
