Effects and Mechanism of Action of Yuebi Decoction on Mouse Glomerular Endothelial Cell Function
- VernacularTitle:越婢汤对小鼠肾小球内皮细胞功能的影响及作用机制
- Author:
Yanru WANG
1
;
Lin XU
1
;
Xiu DU
1
;
Minghai SHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Yuebi Decoction; Oedema; ADR; MGEC; VCAM-1; ICAM-1; Rac1/STAT3
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(3):801-808
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the protective effect of Yuebi Decoction on adriamycin(ADR)-induced mouse glomerular endothelial cells(MGEC),and to explore the possible mechanism of action.Methods MGEC were stimulated with ADR at a concentration of 1.25 μmol·L-1 for 12 h.MGEC were divided into five groups:normal group,model group,and Yuebi Decoction low,medium,and high dosage groups(5%,10%,and 20%of drug-containing serum);the viability of cells was detected by CCK-8 assay,the cell mobility was detected by Transwell assay.The relative expression levels of MGEC VCAM-1,Rac1,STAT3,and p-STAT3 were detected by Western blot.The expression of endothelial marker CD31,VCAM-1 and ICAM-1 was detected by immunofluorescence.Results Compared with the normal group,the endothelial cell migration rate was decreased in the model group(P<0.01),the protein expression levels of VCAM-1,ICAM-1,Rac1 and p-STAT 3 were significantly increased(P<0.05,P<0.01).The endothelial cell migration rate of the high-dose group of Yuebi Decoction was significantly increased compared with that of the model group(P<0.05).VCAM-1,ICAM-1,Rac1 and p-STAT3 protein expression levels of the drug-containing serum of Yuebi Decoction each group were significantly reduced(P<0.05,P<0.01).Conclusion Yuebi Decoction inhibited ADR-induced adhesion molecule expression in MGEC,alleviated endothelial injury,and improved endothelial cell migration rate.Its mechanism may be related to the inhibition of the Rac1/STAT3 signaling pathway.
