Based on Metabolomics,the Mechanism of Qufengxiaoschonlein Decoction in the Treatment of Henoch-Schonlein Purpura was lnvestigated
10.11842/wst.20231129002
- VernacularTitle:基于代谢组学探究祛风消癜方治疗过敏性紫癜的作用机制
- Author:
Hua LIU
1
;
Xianqing REN
;
Weixia LI
;
Qiongqiong XING
;
Leying XI
;
Yifan LI
;
Manxiang YANG
;
Ying DING
Author Information
1. 河南中医药大学 郑州 450046
- Publication Type:Journal Article
- Keywords:
Allergic purpura;
Formula for dispelling wind and eliminating blemishes;
Metabolomics;
Rats;
Ultra high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometer
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(3):612-625
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory effect of Qufeng Xiaodian Formula on serum differential metabolites of allergic purpura,and provide scientific basis for the diagnosis and treatment of allergic purpura in traditional Chinese medicine.Methods Sixty rats were randomly divided into a blank control group(referred to as the blank group),a model group,a compound glycyrrhizin group,a low-dose Qu Feng Xiao Dian Fang group,a medium dose Qu Feng Xiao Dian Fang group,and a high-dose Qu Feng Xiao Dian Fang group according to a random number table method,with 10 rats in each group.The model group was constructed by combining dried ginger,pepper,and long pepper with ovalbumin to create an allergic purpura rat model.After successful modeling,each treatment group was intervened with corresponding drugs for 4 weeks.After 4 weeks,serum was collected and non targeted metabolomics screening of serum differential metabolites was performed using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometer(UPLC-QTOF/MS).Subsequently,data extraction and multivariate statistical analysis will be conducted to identify potential metabolic pathways.Results Compared with the control group,there were 91 possible differential metabolites in the serum of the model group rats,corresponding to 20 metabolic pathways;Compared with the model group,there were a total of 43 possible differential metabolites in the serum of rats in the wind dispelling and disease eliminating group,corresponding to 15 metabolic pathways.Among them,there are a total of 12 metabolic pathways.Inflammatory metabolites such as arachidonic acid and ceramide can damage vascular endothelium.Ten biomarkers,including arachidonic acid and ceramide,were significantly abnormal in the serum of the model group rats compared to the normal group.The Qufeng Xiaodian formula can significantly reverse these metabolites and significantly enrich them in arachidonic acid metabolism and sphingolipid metabolism pathways.Conclusion Qufeng Xiaodian Formula has a certain regulatory effect on metabolites such as arachidonic acid and ceramide that affect vascular endothelial injury.
