miR-204-3p affects silica dust-induced epithelial mesenchymal transition in a rat silicosis model
10.3969/j.issn.1671-7856.2025.03.004
- VernacularTitle:miR-204-3p干预二氧化硅粉尘诱导的大鼠矽肺上皮间质转化进程
- Author:
Jing YU
1
;
Fang CHEN
1
;
Wenxuan HU
1
;
Yangyang PI
1
;
Xi ZHANG
1
;
Luning WANG
1
;
Ping ZHAO
1
;
Faxuan WANG
1
Author Information
1. 宁夏医科大学公共卫生学院,银川 750004
- Publication Type:Journal Article
- Keywords:
silicosis;
silica;
EMT;
ECM;
miR-204-3p
- From:
Chinese Journal of Comparative Medicine
2025;35(3):40-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of miR-204-3p in silicosis and to elucidate the mechanism by which it affects silicosis fibers by regulating silica dust-induced alveolar epithelial-mesenchymal transition(EMT)in rats.Methods Forty SD rats were divided randomly into 4 groups:Control,Silicosis,AAV-Control,and AAV-miR-204-3p groups.The pathology of lung tissue damage was detected by hematoxylin and eosin(HE)and Masson staining.Relative expression levels of miR-204-3p and EMT marker genes in lung tissues from rats in each group were analyzed by real-time fluorescence reverse transcription quantitative PCR(RT-qPCR),and protein expression levels of EMT-related markers in lung tissues were detected by Western blot.Results The alveolar structure was damaged,the lung septa showed interstitial fibrosis,and expression levels of mesenchymal markers were elevated in the Silicosis group compared with the Control group(P＜0.05,P＜0.01,P＜0.001).The alveolar structure was more complete,the EMT process was alleviated,fibrosis was improved,and mesenchymal marker expression was reduced in the AAV-miR-204-3p group compared with the AAV-Control group(P＜0.05,P＜0.01,P＜0.001).Conclusions Free silica dust induces EMT in rat lung tissue.Overexpression of miR-204-3p can attenuate the EMT process induced by free silica dust in rats,and may thus affect silicosis fibrosis.
