Abnormal T cell subsets of BTBR T+Itpr3tf autistic mice at different developmental stages
10.3969/j.issn.1671-7856.2025.03.003
- VernacularTitle:不同发育阶段BTBR T+Itpr3tf孤独症小鼠T细胞亚群异常研究
- Author:
Chen SHEN
1
;
Meng LI
1
;
Zuqing NIE
1
;
Zhiwei LI
1
;
Jie WEN
1
;
Juanjuan DU
1
;
Xiuying KUI
1
;
Jingrui YANG
1
;
Xia CAO
1
Author Information
1. 昆明医科大学第二附属医院实验中心,昆明 650101
- Publication Type:Journal Article
- Keywords:
BTBR T+Itpr3tf mice;
T cell subset;
flow cytometry;
autism
- From:
Chinese Journal of Comparative Medicine
2025;35(3):30-39,47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of T cell subsets in the spleen of BTBR T+Itpr3tf autistic mouse at 4,8,and 12 weeks of age,and to determine the optimal age for studying the relationship between immune abnormalities and autism in BTBR autistic mice.Methods It randomly selected 5～6 male BTBR mouse at 4 weeks,8 weeks,and 12 weeks of age and C57BL/6J mouse of the same gender at corresponding ages for the three-box social interaction test,the self-grooming test,and the marble-burying test;Single cell suspensions were prepared from the spleens of mouse at 8 and 12 weeks of age,and flow cytometry was used to detect 8 subsets of T cells(TH 1,TH2,TH17,TC1,TC2,TC17,TFH,and Treg).Results Compared with C57BL/6J mouse of the same age,BTBR mouse at 4 weeks,8 weeks,and 12 weeks of age showed a decrease in social time(P＜0.001),an increase in grooming time(P＜0.01,P＜0.001),and an increase in the number of marbles buried(P＜0.01,P＜0.001)in BTBR mouse at 8 weeks and 12 weeks of age.As well,the expression of TH 1(P＜0.001),TH2(P＜0.01),TC 1(P＜0.05),TC2(P＜0.001),and TFH(P＜0.01)cells in 8-week-old BTBR mouse were significantly increased,while the expression of Treg(P＜0.001)cells were significantly decreased;The expression of TH 1(P＜0.01),TH2(P＜0.01),TH 17(P＜0.05),TC1(P＜0.01),TC2(P＜0.001),TC 17(P＜0.01),and TFH(P＜0.001)increased in 12-week-old BTBR mouse,while the expression of Treg(P＜0.05)cells decreased.At different age stages(P＜0.050)the ratio of TH 1/Treg and TC 1/Treg in 8-week-old BTBR mouse were significantly higher than those in 12 week old mouse,while the TC 17/Treg ratio decreased.Conclusions BTBR mouse at different developmental stages exhibit varying degrees of abnormal increase in Teff/Treg ratio.Based on result of behavioral test,it is recommended to use 8-week-old BTBR mice for research on autism and immune abnormalities.
