Role of TXNIP in lipid deposition of placental trophoblast in gestational diabetes mellitus
10.13315/j.cnki.cjcep.2025.04.011
- VernacularTitle:TXNIP在妊娠糖尿病胎盘滋养层脂质沉积中的作用
- Author:
Jie YANG
1
;
Jianchao JIA
1
;
Ying ZHANG
1
;
Rina SA
1
;
Dongfang LI
1
;
Zhiying LI
1
;
Na HUANG
1
;
Lan YU
1
Author Information
1. 内蒙古自治区人民医院临床医学研究中心/内蒙古自治区代谢紊乱疾病基因调控重点实验室/内蒙古医学科学院基础研究所,呼和浩特 010017
- Publication Type:Journal Article
- Keywords:
gestational diabetes mellitus;
thioredoxin-interacting protein;
placental trophoblast;
lipid
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(4):483-490
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of thioredoxin-interacting protein(TXNIP)in placental tissues of gestational diabetes mellitus(GDM)and its role in lipid deposition in the placental trophoblast.Methods The pla-centa tissues of 16 GDM pregnant women and 25 women with normal glucose tolerance(NGT)were collected.Mean-while,in vitro models of high-glucose induced human chorionic trophoblast cells(HTR-8/SVneo)were established,which were divided into normal glucose control group(NG),high glucose group(HG),high glucose+si nonsense in-terference group(HG+siNC)and high glucose+TXNIP siRNA group(HG+siTXNIP).The pathological structure and morphological changes of placenta were observed by HE staining.Lipid droplet formation was detected by oil red O staining.The expression and localization of TXNIP in cells were detected by immunocytochemical EnVision method.The mRNA and protein expression levels of TXNIP and lipogenic protein SREBP1 were detected by real-time fluorescent quantitative PCR and Western blot.The relationships between TXNIP expression and lipid droplet accumulation in-duced by high glucose was analyzed.Results High glucose results in abnormal placental structure of GDM.The area of human chorionic intervillous tissue in placenta of GDM group was decreased,the size of fetal capillaries was different and the lumen was dilated.Compared with those NGT group,the accumulation of lipid droplets and the expression of TXNIP mRNA and protein in placental tissue of GDM were increased(P＜0.05).The TXNIP and SREBP1 were up-regulated and the formation of droplets increased in high glucose induced HTR-8/SVneo cells(P＜0.05).On the con-trary,TXNIP siRNA transfection reversed the gene expression level and lipid deposition in high glucose induced cells(P＜0.05).Conclusion TXNIP is involved in abnormal lipid deposition in the placental trophoblast of GDM.
