The relationship between mitochondrial morphology and neuron type in the inferior olive of essential tremor mice
10.16557/j.cnki.1000-7547.2025.02.001
- VernacularTitle:特发性震颤小鼠下橄榄核线粒体形态与神经元类型的关联
- Author:
Ziwei NI
1
;
Hui LIU
;
Jingjing TIE
;
Bozhi LIU
;
Feifei WU
;
Yayun WANG
;
Cailian RUAN
Author Information
1. 延安大学医学院神经生物学教研室,延安 716000
- Publication Type:Journal Article
- Keywords:
essential tremor(ET);
inferior olivary(IO);
mitochondria;
harmaline;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(2):131-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of essential tremor(ET)on the mitochondrial morphology of GABAer-gic and glutamatergic neurons in the inferior olive(IO).Methods:The ET mouse model was established via intraper-itoneal injection of harmaline.Twelve 8-week-old male C57BL/6J mice were randomly divided into saline control group and harmaline treatment group(HA).Behavioral tests,including open field test,rotarod test,balance beam test,and tremor scoring,were conducted to assess the behavior of mice.Using genetic engineering technology and the CRISPR/Cas9 system,we designed and generated 6 male GAD2-Mito-GFP mice and 6 male VGLUT2-Mito-GFP mice,all being 8-week-old.The mice of each transgenic line were randomly divided into Control and HA group.Immunofluorescence staining was used to analyze the expression of c-FOS positive cells in the IO of both the Control and HA groups,and to classify different types of neurons.Mitochondrial network analysis(MiNA)was performed to quantitatively analyze the area,mean aspect ratio,branch length,and other of mitochondria in different types of neurons in the IO under ET con-ditions.Results:Compared to the Control group,the HA group exhibited motor abnormalities and significant tremors.Immunofluorescence results showed a significant increase in the number of c-FOS positive cells in the IO,primarily in GABAergic neurons.MiNA results revealed that the mitochondria of GABAergic neurons showed increased area,branch length diameter,demonstrating irregular morphology.Conclusion:ET induces activation of GABAergic neurons in the IO and leads to more prominent mitochondrial morphological changes.This provides a new perspective for further inves-tigation of the pathogenesis of essential tremor and its relationship with mitochondrial.
