Extraction,culture and identification of primary rabbit brain microvascular endothelial cells in vitro
10.16557/j.cnki.1000-7547.2025.02.010
- VernacularTitle:原代家兔脑微血管内皮细胞的体外提取、培养及鉴定
- Author:
Bolin LI
1
;
Ming CHI
;
Fan ZHANG
;
Yuanyu TANG
Author Information
1. 福建中医药大学:中医学院,福州 350122
- Publication Type:Journal Article
- Keywords:
brain microvascular endothelial cells(BMECs);
primary culture;
identification;
rabbit haemorrhagic disease;
rabbit
- From:
Chinese Journal of Neuroanatomy
2025;41(2):201-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a simple and efficient method for in vitro extraction,culture and identification of brain microvascular endothelial cells(BMECs)of rabbits.Method:The cerebral cortex of 3-week-old rabbits was cut up,sieved and digested with type II collagenase to obtain pure brain microvascular segments for primary culture and identification.Results:The cultured target cells grew in a typical"cobblestone-like"arrangement.Immunohistochemi-cal staining for von Willebrand factor(vWF)related antigen showed that the cytoplasm was presented as brownish red and the expression was positive.Conclusion:The physical sieving method combined with the chemical enzyme diges-tion method can simply and efficiently culture primary rabbit BMECs with strong activity and high purity.
