Puerarin inhibits hydrogen peroxide induced ferroptosis of RSC96 cells through the Nrf2/SLC7A11/GPX4 pathway
10.16557/j.cnki.1000-7547.2025.02.009
- VernacularTitle:葛根素通过Nrf2/SLC7A11/GPX4通路抑制过氧化氢诱导的RSC96细胞铁死亡
- Author:
Jiayin WANG
1
;
Si ZHENG
;
Ming LI
;
Qing ZHU
;
Longju CHEN
Author Information
1. 湖北民族大学医学部解剖教研室,恩施 445000;海南热带海洋学院民族学院,三亚 572022
- Publication Type:Journal Article
- Keywords:
puerarin;
ferroptosis;
Schwann cells(SCs);
Nrf2/SLC7A11/GPX4 signaling pathway
- From:
Chinese Journal of Neuroanatomy
2025;41(2):194-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect and mechanism of puerarin(Pue)on hydrogen peroxide(H2 O2)induced ferroptosis in the rat Schwann cell derived cell line RSC96.Methods:The RSC96 cells were incuba-ted with H2 O2 to establish a cellular injury model,while a subset of cells were co-incubated with H2 O2 and Pue.The vi-ability of cells was examined using the CCK-8 assay.The intracellular levels of glutathione(GSH),total superoxide dismutase(T-SOD),reactive oxygen species(ROS),malondialdehyde(MDA),and ferrous ions(Fe2+)levels were quantified using commercial kits.Western blot was employed to detect the protein expression level of glutathione peroxi-dase 4(GPX4),cyclooxygenase-2(COX-2),solute carrier family 7 member 11(SLC7A11),nuclear factor erythroid 2-related factor 2(Nrf2),and heme oxygenase-1(HO-1).Immunofluorescence assay was performed to examine the expression and nuclear distribution of Nrf2.Results:Pue pretreatment significantly increased the survival rate of H2 O2-treated RSC96 cells,increased the intracellular content of GSH and T-SOD,and inhibited the concentration of ROS and MDA.It also activated the nuclear translocation of Nrf2 and upregulated GPX4,SLC7A11,HO-1,and Nrf2 proteins in RSC96 cells;This effect was abolished by the Nrf2 inhibitor ML385.Conclusion:Pue treatment alleviated the H2 O2-induced ferroptosis of RSC96 cells via the Nrf2/SLC7A11/GPX4 signaling pathway.
