Aromatase-positive astrocytes alleviate cerebral ischemia-reperfusion injury in mice
10.16557/j.cnki.1000-7547.2025.02.003
- VernacularTitle:芳香化酶阳性星形胶质细胞减轻小鼠脑缺血再灌注损伤
- Author:
Tiantian XU
1
;
Yumeng LI
1
;
Yongheng YANG
1
;
Wenqiang ZUO
1
;
Jin WANG
1
;
Shiquan WANG
1
;
Haiyun GUO
1
;
Wugang HOU
1
Author Information
1. 空军军医大学西京医院麻醉与围术期医学科,西安 710032
- Publication Type:Journal Article
- Keywords:
ischemic stroke;
astrocytes;
aromatase(ARO);
neuroprotection;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(2):150-156
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of reactive astrocytes expressing aromatase(ARO)in the penumbra during ischemic stroke.Methods:A mouse model of middle cerebral artery occlusion(MCAO/R)was prepared using the suture method.Western blot and immunofluorescence staining were used to observe the expression of ARO in the penumbra after ischemia reperfusion.We generated a glial fibrillary acidic protein promoter-driven aromatase knock-out(GFAP-ARO-KO)mouse model in vivo.Neurologic impairment scores,rotarod test,grip strength test and adhesive removal test on the plantar surface of the paw were performed after MCAO/R modeling.Results:In wild type mice,the expression of ARO was significantly increased in astrocytes in the penumbra after MCAO/R(P<0.01).Compared to the control group,the expression of ARO in the GFAP-ARO-KO group was significantly reduced in astrocytes in the penumbra after MCAO/R.And loss of ARO increased cerebral infarction volume and aggravated sensorimotor impair-ment.Conclusion:After ischemic stroke,reactive astrocytes in the penumbra highly express ARO and play a protective role in post-ischemia reperfusion injury.
