Effect of fibrinogen-like protein 2 on immune response of EBV-infected T lymphocyte
10.13431/j.cnki.immunol.j.20250002
- VernacularTitle:纤维蛋白原样蛋白2对EBV感染的T细胞免疫应答的影响研究
- Author:
Yuzhen HONG
1
;
Min LI
;
Bing CHENG
;
Yueping LIU
;
Bo DIAO
Author Information
1. 430065,武汉科技大学医学部医学院;430070 武汉,中部战区总医院基础医学实验室
- Publication Type:Journal Article
- Keywords:
EBV;
FGL2;
T-cell Exhaustion;
T-cell Activation;
MOI
- From:
Immunological Journal
2025;41(1):16-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aims to investigate the effects of FGL2 on the immune response of EBV-infected T cells,including their activation,proliferation,exhaustion,and cytokine profile changes.Methods Primary T cells were infected with EBV at different multiplicities of infection(MOI).Expression of FGL2 in T cells,as well as T-cell activation,proliferation,exhaustion,and cytokine levels,were detected using RT-qPCR,Western blot,ELISA,CCK8,and flow cytometry(FCM),respectively.Further experiments involving FGL2 knockdown and overexpression were conducted to elucidate its specific regulatory role in EBV-infected T cells.Results FGL2 expression was significantly upregulated in EBV-infected T cells(P＜0.05).EBV infection also induced enhanced T cell activation(P＜0.001),proliferation(P＜0.001),and exhaustion(P＜0.01).Compared to the T cells+EBV group,the T cells+EBV+FGL2 overexpression group exhibited higher exhaustion levels(P＜0.01),reduced activation(P＜0.05)and proliferation(P＜0.05),decreased pro-inflammatory cytokine levels(P＜0.05),and increased anti-inflammatory cytokine levels(P＜0.05).Conversely,the T cells+EBV+FGL2 knockdown group demonstrated the opposite trends,with elevated activation(P＜0.01),proliferation(P＜0.05),pro-inflammatory cytokine levels(P＜0.05),and reduced exhaustion(P＜0.01)and anti-inflammatory cytokine levels(P＜0.05).Conclusion FGL2 suppresses T cell activation and proliferation,exacerbates T cell exhaustion,inhibits pro-inflammatory cytokine release,and promotes anti-inflammatory cytokine secretion during EBV infection,thereby modulating the immune response of T cells.
